NPT antibody

Catalog No. ABIN966694

Product Details

Target: NPT

Reactivity: Human

Host: Please inquire

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: ELISA, Western Blotting (WB)

Specificity: Ni-NTA purified truncated recombinant NPT expressed in E. Coli strain BL21 (DE3)

Purification: Antibodies are purified by protein A affinity chromatography

Isotype: IgG2a

Application Details

Application Notes: Western Blot: Dilution 1: 500- 1: 2,000
ELISA: Propose dilution 1: 10,000.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Storage: -20 °C

References

Stewart, Richards, Halfhill: "Transgenic plants and biosafety: science, misconceptions and public perceptions." in: BioTechniques, Vol. 29, Issue 4, pp. 832-6, 838-43, (2001) (PubMed).

Target Details

Target: NPT

Background: Neomycin phosphotransferase II (nptII) gene is used in selection of transformed organisms. It was initially isolated from the transposon Tn5 that was present in the bacterium strain Escherichia coli K12. The gene codes for the aminoglycoside 3'-phosphotransferase (denoted aph(3')-II or NPTII) enzyme. NPTII is probably the most widely used selectable marker for plant transformation. It is also used in gene expression and regulation studies in different organisms in part because N-terminal fusions can be constructed that retain enzymatic activity. In animal cells, G418 and neomycin are used as selectable agents.