BTAF1 antibody

Catalog No. ABIN933089

The Mouse Monoclonal anti-BTAF1 antibody has been validated for IP, WB, FACS, ICC and DB. It is suitable to detect BTAF1 in samples from Human.

Quick Overview for BTAF1 antibody (ABIN933089)

Target: BTAF1 (TBP-Associated Factor 172 (BTAF1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This BTAF1 antibody is un-conjugated

Application: Immunoprecipitation (IP), Western Blotting (WB), Flow Cytometry (FACS), Immunocytochemistry (ICC), Dot Blot (DB)

Clone: BTA3D61

Product Details anti-BTAF1 Antibody

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details): Other species not studied.

Purification: Protein G affinity chromatography

Immunogen: BTAF1 antibody was raised in mouse using recombinant Btaf1 Rna Polymerase Ii, B-Tfiid Transcription Factor-Associated, 170 Kda (Mot1 Homolog, S. Cerevisiae) (Btaf1)

Isotype: IgG1

Application Details

Application Notes: WB: 0.2-2 µg/mL, IP: 100-500 µg/sample, FC: 0.5-2 µg/sample, ICC: 2-100 µg/mL
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: Lot specific

Buffer: BTAF1 antibody in PBS (3.0 mM KCl, 1.5 mM KH2 PO4 , 140 mM NaCl, 8.0 mM Na2 HPO4 (pH 7.4)) containing 1 % bovine serum albumin (BSA) and 0.05 % sodium azide (NaN3).

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2-8 °C for up to one year. We recommend long term storage at -20 °C.

Target Details for BTAF1

Target: BTAF1 (TBP-Associated Factor 172 (BTAF1))

Alternative Name: BTAF1

Background: Initiation of transcription by RNA polymerase II requires the assistance of TATA box-binding protein (TBP, MIM 600075) and TBP-associated factors, or TAFs (e.g., TAF2B, MIM 604912), in 2 distinct complexes, TFIID and B-TFIID. The TFIID complex is composed of TBP and more than 8 TAFs. However, the majority of TBP is present in the B-TFIID complex, which is composed of TBP and TAFII170, also called TAF172, and has DNA-dependent ATPase activity. Synonyms: Monoclonal BTAF1 antibody, Anti-BTAF1 antibody, B-TFIID transcription factor-associated, MOT1 antibody, TAF172 antibody, KIAA0940 antibody, TAFII170 antibody, TAF(II)170 antibody.