beta Actin antibody (N-Term)

Catalog No. ABIN933342

This anti-beta Actin antibody is a Mouse Monoclonal antibody detecting beta Actin in WB, ELISA and DB. Suitable for Human.

Quick Overview for beta Actin antibody (N-Term) (ABIN933342)

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This beta Actin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Dot Blot (DB)

Clone: BA3R

Product Details anti-beta Actin Antibody

Binding Specificity: N-Term

Cross-Reactivity: Mouse (Murine), Rat (Rattus), Rabbit, Chicken

Purification: Protein A affinity chromatography

Immunogen: beta Actin antibody was raised in mouse using beta-Actin N-terminal peptide-KLH conjugates as the immunogen.

Isotype: IgG2b

Application Details

Application Notes: WB: 1:1000-1:3000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: Supplied as a liquid in 10 mM PBS, pH 7.2, with 0.05 % NaN3

Preservative: Sodium azide

Precaution of Use: This product contains Sodium Azide: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C for several days to weeks. Add 0.1 % NaN3 and store at -20 °C for longer periods.

Target Details for beta Actin

Target: beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name: beta Actin

Background: Beta-actin is one of six different actin isoforms which have been identified in humans. This is one of the two nonmuscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Alpha actins are a major constituent of the contractile apparatus. Beta actin is usually used as a loading control, for among others, the integrity of cells, protein degradation, in Western Blotting. Synonyms: Monoclonal beta Actin antibody, anti beta Actin antibody, B Actin antibody, BA3R antibody, beta Actin Loading Control antibody, ATCB antibody.

Pathways: Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction