Nef antibody

Catalog No. ABIN933455

The Mouse Monoclonal anti- antibody has been validated for WB and ELISA. It is suitable to detect in samples from Human Immunodeficiency Virus (HIV).

Quick Overview for Nef antibody (ABIN933455)

Target: Nef

Reactivity: Human Immunodeficiency Virus (HIV)

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), ELISA

Clone: 2H12

Product Details

Purity: > 95 % pure

Immunogen: HIV1 Nef antibody was raised in mouse using full length recombinant nef (HIV-1) as the immunogen.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1:1,000, WB: 1:1,000
Optimal conditions should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Reconstitute in sterile distilled water.

Concentration: Lot specific

Buffer: Supplied lyophilized from PBS buffer, with no preservatives added

Preservative: Without preservative

Handling Advice: Avoid repeated freeze/thaw cycles.
Dilute only prior to immediate use.

Storage: 4 °C/-20 °C

Storage Comment: Store at 4 °C until reconstitution. Following reconstitution aliquot and freeze at -70 °C for long term storage.

Target Details

Target: Nef

Target Type: Viral Protein

Background: Nef (Negative Regulatory Factor) is a protein expressed by primate lentiviruses. These include human immunodeficiency viruses (HIV-1 and HIV-2) and simian immunodeficiency virus (SIV). Nef is one of many pathogen-expressed proteins, known as virulence factors, which function to manipulate the host's cellular machinery and thus allow infection, survival or replication of the pathogen. Nef stands for Negative Factor. Synonyms: Monoclonal HIV1 Nef antibody, Anti-HIV1 Nef antibody, HIV-1 Nef antibody, 3'ORF antibody, ABIN antibody, ABIN1 antibody,C terminal core protein antibody, F protein antibody, Nef antibody, Negative factor antibody, p27 antibody, VAN antibody.