SEMA7A antibody (Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group))

Details for Product anti-SEMA7A Antibody No. ABIN93945
Antigen
Reactivity
Human
86
53
34
2
1
1
1
1
Host
Mouse
56
33
2
1
Clonality (Clone)
Monoclonal ()
Conjugate
This SEMA7A antibody is un-conjugated
8
4
3
3
3
3
3
3
2
1
1
1
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
59
29
24
21
13
10
8
5
3
2
1
Options
Immunogen HPB-ALL human T cell line
Clone MEM-150
Isotype IgM
Specificity The antibody MEM-150 reacts with CD108 (JMH blood group antigen), a 80 kDa GPI-anchored glycoprotein expressed on various cell types including erythrocytes, lymphoblasts, at low levels it is present on circulating lymphocytes.
HLDA V, WS Code AS S017
HLDA V, WS Code BP BP347
HLDA VI, WS Code BP 401
HLDA VI, WS Code BP 475
HLDA VI, WS Code NL N-L156
HLDA VI, WS Code P PR-65
Cross-Reactivity (Details) Human
Purification Purified by precipitation and chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD108 (SEMA7A Antibody Abstract)
Background CD108 (Sema7A) is a GPI-anchored semaphorin family member, which enhances central and peripheral axonal growth and is required for proper axon track formation during ebryogenesis. CD108 also regulates osteoclast differentiation and pre-osteoblastic cell migration, and in immune system affects cell proliferation, chemotaxis and cytokine release. On erythrocytes CD108 defines the JMH (John-Milton-Hagen) human blood group. CD108 signalizes through its receptors –, plexin C1 and beta1 integrins.
Pathways Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Regulation of Cell Size
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Tris buffered saline (TBS) with 15 mM sodium azide, approx. pH 8.0
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Semaphorin 7A, GPI Membrane Anchor (John Milton Hagen Blood Group) (SEMA7A) antibody (ABIN93945) Surface staining of HPB-ALL human peripheral blood T cell leukemia cell line with ant...
Product cited in: Angelisová, Drbal, Cerný, Hilgert, Horejsí: "Characterization of the human leukocyte GPI-anchored glycoprotein CDw108 and its relation to other similar molecules." in: Immunobiology, Vol. 200, Issue 2, pp. 234-45, 1999 (PubMed).

Mudad, Rao, Angelisova, Horejsi, Telen: "Evidence that CDw108 membrane protein bears the JMH blood group antigen." in: Transfusion, Vol. 35, Issue 7, pp. 566-70, 1995 (PubMed).