CD14 antibody

Catalog No. ABIN93977

Product Details anti-CD14 Antibody

Target: CD14

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD14 antibody is un-conjugated

Application: Flow Cytometry (FACS), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: The mouse monoclonal antibody B-A8 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol)-linked extracellular membrane glycoprotein expressed on the surface of monocytes, macrophages and weakly on granulocytes, also expressed by most tissue macrophages.

Cross-Reactivity (Details): Human

Purification: Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods).

Purity: > 95 % (by SDS-PAGE)

Immunogen: Human monocytes

Clone: B-A8

Isotype: IgG1

Application Details

Application Notes: ELISA: The antibody B-A8 has been tested as the capture antibody in a sandwich ELISA for analysis of human CD14 in combination with antibody MEM-18 (cat. no. 1B-212-C100). Flow cytometry: recommended dilution: 1 μg/mL, Immunohistochemistry (paraffin sections): recommended dilution: 2-10 μg/mL, positive tissue: PML brain sections, positive staining on human PML brain sections was mainly observed on monocytes in the lumenal side of brain blood vessels, and on some perivascular cells adjacent to medium-sized vessels. Heat retrieval of antigen is recommeded.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

References for anti-CD14 antibody (ABIN93977)

Yates, Rovis, Mitchell, Afzali, Tsang, Garin, Lechler, Lombardi, Garden: "The maintenance of human CD4+ CD25+ regulatory T cell function: IL-2, IL-4, IL-7 and IL-15 preserve optimal suppressive potency in vitro." in: International immunology, Vol. 19, Issue 6, pp. 785-99, (2007) (PubMed).

Kuusniemi, Lapatto, Holmberg, Karikoski, Rapola, Jalanko: "Kidneys with heavy proteinuria show fibrosis, inflammation, and oxidative stress, but no tubular phenotypic change." in: Kidney international, Vol. 68, Issue 1, pp. 121-32, (2005) (PubMed).

Matasić, Dietz, Vuk-Pavlović: "Dexamethasone inhibits dendritic cell maturation by redirecting differentiation of a subset of cells." in: Journal of leukocyte biology, Vol. 66, Issue 6, pp. 909-14, (2000) (PubMed).

Goyert, Ferrero, Rettig, Yenamandra, Obata, Le Beau: "The CD14 monocyte differentiation antigen maps to a region encoding growth factors and receptors." in: Science (New York, N.Y.), Vol. 239, Issue 4839, pp. 497-500, (1988) (PubMed).

Target Details for CD14

Target: CD14

Alternative Name: CD14 (CD14 Products)

Synonyms: CD14 molecule antibody, CD14 antigen antibody, CD14 antibody, Cd14 antibody

Background: CD14 Molecule,CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.,LPS-receptor, LPSR, MCLR

Gene ID: 929

UniProt: P08571

Pathways: TLR Signaling, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades