CD235ab antibody (N-Term)

Catalog No. ABIN94041

This anti-CD235ab antibody is a Mouse Monoclonal antibody detecting CD235ab in FACS, IHC (fro), AGG and IHC (p). Suitable for Human.

Quick Overview for CD235ab antibody (N-Term) (ABIN94041)

Target: CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235ab antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Aggregation (AGG), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: HIR2

Product Details anti-CD235ab Antibody

Binding Specificity: N-Term

Purpose: Anti-Hu CD235ab Purified

Specificity: The mouse monoclonal antibody HIR2 recognizes an epitope within the N-terminal (extracellular) portion of CD235a (glycophorin A) and CD235b (glycophorin B). Its antigen is expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL, but not on all other cells.

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Synthetic peptide (Human, N-terminal)

Isotype: IgG2b

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD235ab

Target: CD235ab (CD235a + CD235b) (Glycophorin A + B (CD235a + CD235b))

Alternative Name: CD235ab

Background: CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A/B, GYPA/B, GPA/B, PAS-2/3, Sialoglycoprotein alpha/beta, GPSAT, GPErik, MSN blood group