CD46 Molecule, Complement Regulatory Protein (CD46) antibody

Details for Product No. ABIN94149
Antigen
  • AHUS2
  • MCP
  • MIC10
  • TLX
  • TRA2.10
  • Mcp
  • CD46 molecule
  • CD46 antigen, complement regulatory protein
  • CD46 molecule, complement regulatory protein
  • membrane cofactor protein-like
  • CD46
  • Cd46
  • LOC100341359
Reactivity
Human, Cow (Bovine)
293
51
34
32
26
8
Host
Mouse
222
128
2
Clonality (Clone)
Monoclonal ()
Conjugate
Un-conjugated
33
26
22
15
13
8
4
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
Application
Flow Cytometry (FACS), Immunoprecipitation (IP), Western Blotting (WB)
216
177
116
73
61
59
33
16
14
10
9
8
8
2
2
1
Options
Immunogen HPB-ALL human T cell line
Clone MEM-258
Isotype IgG1
Specificity The antibody MEM-258 recognizes an epitope on SCR4 (the membrane-proximal SCR) domain of CD46 (Membrane cofactor protein). CD46 is 56-66 kDa dimeric transmembrane protein expressed on T and B lymphocytes, platelets, monocytes, granulocytes, endothelial cells, epithelial cells and fibroblast, it is negative on erythrocytes.
Cross-Reactivity (Details) Human, Bovine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD46 (CD46 Antibody Abstract)
Background CD46 (MCP, membrane cofactor protein) is a multifunctional cell surface transmembrane protein that binds and inactivates C3b and C4b complement fragments, regulates T cell-induced inflammatory responses by either inhibiting (CD46-1 isoform) or increasing (CD46-2 isoform) the contact hypersensitivity reaction. CD46 also serves as a receptor for several human pathogens (both bacteria and viruses), and its ligation alteres T lymphocyte polarization toward antigen-presenting cells or target cells, inhibiting lymphocyte function. CD46 is a protector of placental tissue and is also expressed on the inner acrosomal membrane of spermatozoa.
Pathways Regulation of Actin Filament Polymerization
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-CD46 Molecule, Complement Regulatory Protein (CD46) antibody (ABIN94149) Surface staining of human peripheral blood cells with anti-CD46 (MEM-258) PE.
Product cited in: Kälin, Amstutz, Gastaldelli, Wolfrum, Boucke, Havenga, DiGennaro, Liska, Hemmi, Greber: "Macropinocytotic uptake and infection of human epithelial cells with species B2 adenovirus type 35." in: Journal of virology, Vol. 84, Issue 10, pp. 5336-50, 2010 (PubMed).

Wang, Tuve, Erdman, Lieber: "Receptor usage of a newly emergent adenovirus type 14." in: Virology, Vol. 387, Issue 2, pp. 436-41, 2009 (PubMed).

Hoffmann, Bayer, Heim, Potthoff, Nettelbeck, Wildner: "Evaluation of twenty-one human adenovirus types and one infectivity-enhanced adenovirus for the treatment of malignant melanoma." in: The Journal of investigative dermatology, Vol. 128, Issue 4, pp. 988-98, 2008 (PubMed).

Fleischli, Sirena, Lesage, Havenga, Cattaneo, Greber, Hemmi: "Species B adenovirus serotypes 3, 7, 11 and 35 share similar binding sites on the membrane cofactor protein CD46 receptor." in: The Journal of general virology, Vol. 88, Issue Pt 11, pp. 2925-34, 2007 (PubMed).

Loré, Adams, Havenga, Precopio, Holterman, Goudsmit, Koup: "Myeloid and plasmacytoid dendritic cells are susceptible to recombinant adenovirus vectors and stimulate polyfunctional memory T cell responses." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 179, Issue 3, pp. 1721-9, 2007 (PubMed).

Fremeaux-Bacchi, Moulton, Kavanagh, Dragon-Durey, Blouin, Caudy, Arzouk, Cleper, Francois, Guest, Pourrat, Seligman, Fridman, Loirat, Atkinson: "Genetic and functional analyses of membrane cofactor protein (CD46) mutations in atypical hemolytic uremic syndrome." in: Journal of the American Society of Nephrology : JASN, Vol. 17, Issue 7, pp. 2017-25, 2006 (PubMed).

Weyand, Lee, Higashi, Cawley, Yoshihara, So: "Monoclonal antibody detection of CD46 clustering beneath Neisseria gonorrhoeae microcolonies." in: Infection and immunity, Vol. 74, Issue 4, pp. 2428-35, 2006 (PubMed).

Fleischli, Verhaagh, Havenga, Sirena, Schaffner, Cattaneo, Greber, Hemmi: "The distal short consensus repeats 1 and 2 of the membrane cofactor protein CD46 and their distance from the cell membrane determine productive entry of species B adenovirus serotype 35." in: Journal of virology, Vol. 79, Issue 15, pp. 10013-22, 2005 (PubMed).

Background publications Oliaro, Pasam, Waterhouse, Browne, Ludford-Menting, Trapani, Russell: "Ligation of the cell surface receptor, CD46, alters T cell polarity and response to antigen presentation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 103, Issue 49, pp. 18685-90, 2006 (PubMed).

Liszewski, Kemper, Price, Atkinson: "Emerging roles and new functions of CD46." in: Springer seminars in immunopathology, Vol. 27, Issue 3, pp. 345-58, 2005 (PubMed).

Gaggar, Shayakhmetov, Lieber: "CD46 is a cellular receptor for group B adenoviruses." in: Nature medicine, Vol. 9, Issue 11, pp. 1408-12, 2003 (PubMed).

Marie, Astier, Rivailler, Rabourdin-Combe, Wild, Horvat: "Linking innate and acquired immunity: divergent role of CD46 cytoplasmic domains in T cell induced inflammation." in: Nature immunology, Vol. 3, Issue 7, pp. 659-66, 2002 (PubMed).

Okada, Liszewski, Atkinson, Caparon: "Membrane cofactor protein (CD46) is a keratinocyte receptor for the M protein of the group A streptococcus." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, Issue 7, pp. 2489-93, 1995 (PubMed).