TNFRSF10B antibody (Tumor Necrosis Factor Receptor Superfamily, Member 10b) (Extracellular Domain)

Details for Product anti-TNFRSF10B Antibody No. ABIN94304
Antigen
Epitope
Extracellular Domain
25
14
13
10
9
9
8
8
8
7
5
4
4
4
4
3
3
3
3
3
3
3
2
2
2
2
1
1
1
1
1
1
1
Reactivity
Human
280
58
27
1
1
1
Host
Mouse
182
122
6
2
2
Clonality (Clone)
Monoclonal ()
Conjugate
This TNFRSF10B antibody is un-conjugated
17
16
11
5
5
4
3
3
3
3
3
3
2
2
2
2
2
2
1
Application
Flow Cytometry (FACS)
174
97
71
56
42
33
30
28
13
11
11
5
3
3
2
2
1
Options
Immunogen Recombinant fusion protein of human IgG heavy chain and extracellular domain of DR5.
Clone DR5-01-1
Isotype IgG1
Specificity The antibody DR5-01-1 recognizes an extracellular domain of TRAIL-R2 (DR5). TRAIL-R2 is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L).
Cross-Reactivity (Details) Human
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name CD262 / TRAIL-R2 (TNFRSF10B Antibody Abstract)
Background TRAIL-R2 (CD262, DR5) is one of two TNF superfamily member intracellular death domain containing receptors for TRAIL (APO2L). Apoptosis, or programmed cell death, occurs during normal cellular differentiation and development of multicellular organisms. Apoptosis is induced by certain cytokines including tumor necrosis factor (TNF) and Fas ligand in the TNF family through their death domain containing receptors, TNF receptor 1 (TNFR1) and Fas, respectively. Another member in the TNF family has been identified and designated TRAIL (for TNF related apoptosis inducing ligand) and Apo2L (for Apo2 ligand). Receptors for TRAIL include two death domain containing receptors, DR4 and DR5, as well as two decoy receptors, DcR1 and DcR2, lacking the intracellular signaling death domain. DcR1 (also called TRID), like the related death receptors DR4 and DR5, contains two extracellular cysteine rich domains. However, DcR1 contains no intracellular death domain and is thus incapable of signaling apoptosis. It has been suggested DcR1 is responsible for TRAIL resistance in normal human tissues including heart, placenta, lung, liver, kidney, spleen, and bone marrow. DR5 is a member of the TNF receptor superfamily, and contains an intracellular death domain. This receptor can be activated by tumor necrosis factor related apoptosis inducing ligand (TNFSF10/TRAIL/APO2L), and transduces apoptosis signal. Studies with FADD deficient mice suggested that FADD, a death domain containing adaptor protein, is required for the apoptosis mediated by this protein.
Pathways p53 Signaling, Apoptosis, Positive Regulation of Endopeptidase Activity
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.