IgE antibody

Catalog No. ABIN94399

This Mouse Monoclonal antibody specifically detects IgE in ELISA, WB and FACS. It exhibits reactivity toward Human.

Quick Overview for IgE antibody (ABIN94399)

Target: IgE

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IgE antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS)

Clone: 4H10

Product Details anti-IgE Antibody

Purpose: Anti-Hu IgE Purified

Specificity: The mouse monoclonal antibody 4H10 reacts with human IgE, it recognizes an epitope different from the ones recognized by BE5 and 4G7.325 antibodies to IgE. The epitope is located within the amino acids 267-279 (TWLEDGQVMDVDL).

Cross-Reactivity (Details): Human

Purification: Purified by protein-A affinity chromatography.

Purity: > 95 % (by SDS-PAGE)

Immunogen: Purified human IgE.

Isotype: IgG1

Application Details

Application Notes: Flow cytometry: Recommended dilution: 4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for IgE

Target: IgE

Background: Immunoglobulin E (IgE) is a 180 kDa soluble protein serving as an antigen-specific unit of mast cell effector mechanisms. IgE has the lowest serum concentration of all immunoglobulins (approximately 0.5 mg/l) in healthy individuals, but upon allergen challenge its concentration in blood increases dramatically. Although biological survival of free IgE is very short (T1/2 = 2 days), it is stabilized after binding to its high affinity receptor. Unlike IgM- IgG- and IgA-committed B cells, IgE-switched B cells do not undergo clonal expansion.,Immunoglobulin E

Molecular Weight: 180 kDa