CDK1 antibody (Cyclin-Dependent Kinase 1)

Details for Product anti-CDK1 Antibody No. ABIN94446
Antigen
Reactivity
Human, Non-Human Primate, Cow (Bovine)
665
373
340
37
36
30
25
17
17
15
15
11
10
9
9
8
4
4
3
2
2
1
1
1
1
1
1
1
1
1
Host
Mouse
545
147
Clonality (Clone)
Monoclonal ()
Conjugate
This CDK1 antibody is un-conjugated
35
21
20
15
14
14
13
13
13
13
13
13
5
5
5
5
5
3
3
Application
Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunoprecipitation (IP), Western Blotting (WB)
549
250
197
102
99
75
66
59
32
20
12
9
7
7
6
5
3
2
2
2
2
1
1
1
Options
Immunogen Bacterially expressed full-length human Cdk1 protein
Clone POH-1
Isotype IgG2a
Specificity The antibody POH-1 specifically detects Cdk1 (p34Cdc2). Staining of Cdk1 reflects the proliferating potential of respective tissue.
No Cross-Reactivity Mouse (Murine), Rat (Rattus), Xenopus laevis, Fruit Fly (Drosophila melanogaster)
Cross-Reactivity (Details) Human, Non-Human Primates, Bovine
Purification Purified by protein-A affinity chromatography
Purity > 95 % (by SDS-PAGE)
Antigen
Alternative Name Cdk1 (CDK1 Antibody Abstract)
Background Cdk1 (cyclin-dependent kinase 1), also known as p34Cdc2 (cell division control protein kinase 2) depends on cyclin A and B and is triggered by a positive feedback loop at the end of G2 phase, which is the key event that initiates mitotic entry. Destruction of cyclin B during metaphase results in inactivation of Cdk1, allowing mitotic exit and cell division. Cdk1 also contributes to the control of DNA replication. Cdk1 can be ihibited by several transcriptional targets of p53, such as p21WAF.
Pathways Cell Division Cycle, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Mitotic G1-G1/S Phases, DNA Replication, M Phase, Toll-Like Receptors Cascades, Synthesis of DNA
Application Notes Optimal working dilution should be determined by the investigator.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
Preservative Sodium azide
Precaution of Use This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Do not freeze.
Storage 4 °C
Storage Comment Store at 2-8°C. Do not freeze. Do not use after expiration date stamped on vial label.
Supplier Images
Image no. 1 for anti-Cyclin-Dependent Kinase 1 (CDK1) antibody (ABIN94446) Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 (cl...
Product cited in: Lukás, Draetta, Bartek: "Distinct forms of human CDC2 identified by novel monoclonal antibodies." in: European journal of biochemistry / FEBS, Vol. 207, Issue 1, pp. 169-76, 1992 (PubMed).