Intestinal Alkaline Phosphatase antibody

Catalog No. ABIN94930

Supplier Product No.: 200-1135-0100

The Goat Polyclonal anti-Intestinal Alkaline Phosphatase antibody has been validated for WB and ELISA. It is suitable to detect Intestinal Alkaline Phosphatase in samples from Cow.

Quick Overview for Intestinal Alkaline Phosphatase antibody (ABIN94930)

Target: Intestinal Alkaline Phosphatase (ALPI)

Reactivity: Cow

Host: Goat

Clonality: Polyclonal

Conjugate: This Intestinal Alkaline Phosphatase antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-Intestinal Alkaline Phosphatase Antibody

Supplier Product No.: 200-1135-0100

Supplier: Rockland

Purpose: Alkaline Phosphatase Antibody

Cross-Reactivity (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum as well as purified and partially purified Alkaline Phosphatase [Calf Intestine].

Characteristics: Synonyms: goat anti-Alkaline Phosphatase Antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody

Purification: Anti-ALKALINE PHOSPHATASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogen:

Immunogen: Alkaline Phosphatase [Calf Intestine]

Immunogen Type: Native Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: Anti-Alkaline Phosphatase has been assayed against 1.0 μg of Alkaline Phosphatase [Calf intestine] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2'-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:8,000 of the reconstitution concentration is suggested for this product.

Western Blot Dilution: 1:500 - 1:2,000

ELISA Dilution: 1:4,000 - 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Expiry Date: 12 months

Target Details for Intestinal Alkaline Phosphatase

Target: Intestinal Alkaline Phosphatase (ALPI)

Alternative Name: Calf Intenstinal Alkaline Phosphatase

Background: Background: In most mammals there are four different isozymes: placental, placental-like, intestinal and tissue non-specific (liver/bone/kidney). Alkaline Phosphatase catalytic activity is phosphate monoester + H2O = an alcohol + phosphate.

Gene ID: 280993

UniProt: P19111