ACPP antibody

Catalog No. ABIN950249

The Chicken Polyclonal anti-ACPP antibody has been validated for IF and IHC (p). It is suitable to detect ACPP in samples from Mouse.

Quick Overview for ACPP antibody (ABIN950249)

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Reactivity: Mouse

Host: Chicken

Clonality: Polyclonal

Conjugate: This ACPP antibody is un-conjugated

Application: Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-ACPP Antibody

Specificity: Recognizes Mouse Prostatic Acid Phosphatase (PAP).

Purification: Affinity Chromatography using a peptide column.

Immunogen: Recombinant Mouse PAP protein was expressed using a baculoviral-delivery system. Preparation: After repeated injections, immune eggs were collected from laying hens, from which IgY antibody were prepared (“anti-PAP IgY fraction”). Some of this antibody was further purified using an agarose matrix to which the PAP protein was convalently attached (“Affinity-purified anti-PAP”). The final preparation in the accompanying vial contains 10 mg/ml of the “anti-PAP IgY fraction” supplemented with 20 mg/ml of the “affinity-purified anti-PAP” plus 50% (v/v) Glycerol (to prevent freezing at –20°C). Finally, this antibody preparation was filter-sterilized (0.45 mm) and 200 µl aliquots prepared.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 10 mg IgY/ml (based on absorbance at 280 nm)

Buffer: 10 mM PBS, pH 7.2 containing 1 % BSA as stabilizer and 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for ACPP

Target: ACPP (Acid Phosphatase, Prostate (ACPP))

Alternative Name: ACPP / Prostatic Acid Phosphatase

Background: Mouse PAP is a 43,698 dalton protein (381 amino acids, NCBI accession numberAAF23171) associated with prostatic cancer cells, as well as primary afferent sensory neurons involved in the pain pathway. This protein is an enzyme that dephosphorylates adenosine monophosphate (AMP) in the dorsal horn gray matter of the spinal cord, generating free adenosine. Injections of PAP into the dorsal horn of experimental mice has been shown to decrease pain perception by acting in an antinociceptive, antihyperalgesic, and antiallodynic fashion.Synonyms: ACP3, PAP, PSAP, Prostate acid phosphatase

Gene ID: 56318

NCBI Accession: NP_062781

Pathways: Synaptic Membrane, Ribonucleoside Biosynthetic Process