CD3 antibody (Biotin)

Catalog No. ABIN951289

Product Details anti-CD3 Antibody (Biotin)

Target: CD3

Reactivity: Mouse

Host: Hamster

Clonality: Monoclonal

Conjugate: This CD3 antibody is conjugated to Biotin

Application: Flow Cytometry (FACS)

Specificity: This anti-mouse CD3ε monoclonal antibody is specific for the ε chain of mouse CD31. In the mouse, CD3 is highly expressed by mature T cells and at lower levels by CD4+CD8+ thymocytes2. The ε chain of CD3 is a component of the multimeric T cell receptor complex and is involved with signal transduction. When immobilized this antibody can be used to stimulate mouse T cells in vitro1. A combination of this antibody and anti-mouse CD28 mAb is commonly used for the activation of mouse T cells3.

Clone: 500-A2

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL

Buffer: PBS, 0.1 % NaN3 and EIA grade BSA as stabilizing protein to bring the total protein concentration to 4-5 mg/mL

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C. DO NOT FREEZE! Should this product contain a precipitate we recommend microcentrifugation before use.

Target Details for CD3

Target: CD3

Alternative Name: CD3 (CD3 Products)

Background: T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits: CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82 % of normal peripheral blood lymphocytes, 65-85 % of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.Synonyms: T-cell surface antigen T3/Leu-4, T-cell surface glycoprotein CD3, T3/Leu-4

Gene ID: 12501

NCBI Accession: NP_031674

UniProt: P22646

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway