GAD antibody (C-Term)

Catalog No. ABIN952421

The Chicken Polyclonal anti-GAD antibody has been validated for WB, IHC (p) and IF. It is suitable to detect GAD in samples from Human, Mouse and Rat.

Quick Overview for GAD antibody (C-Term) (ABIN952421)

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Reactivity: Human, Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This GAD antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunofluorescence (IF)

Product Details anti-GAD Antibody

Binding Specificity: C-Term

Specificity: Recognizes Glutamic Acid Decarboxylase (GAD1/GAD67).

Purification: Affinity Chromatography using a peptide column.

Immunogen: Synthetic peptide KLH conjugated corresponding to a region near the C-terminus of this gene product, and was 100% conserved between the Human (Q99259), Mouse (P48318) and Rat (NP_058703) gene products. After repeated injections into the hens, immune eggs were collected, and the IgY fractions were purified from the yolks.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.1 mg/mL (based on Bradford assay readings using BSA as a standard)

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for GAD

Target: GAD (GAD1) (Glutamate Decarboxylase 1 (Brain, 67kDa) (GAD1))

Alternative Name: GAD1 / GAD67

Background: Human Glutamic Acid Decarboxylase (GAD-67), [EC 4.1.1.15] is a 66,987 dalton protein (594 amino acids) selectively expressed in a subpopulation of GABAergic neurons of the CNS. It catalyzes the decarboxylation of glutamic acid, forming the inhibitory neurotransmitter β-amino butyric acid (GABA). It is also know as GAD-1.Synonyms: 67 kDa glutamic acid decarboxylase, GAD-67, Glutamate decarboxylase 1, Glutamate decarboxylase 67 kDa isoform

Gene ID: 2571

NCBI Accession: NP_000808