Nestin antibody

Catalog No. ABIN953652

This anti-Nestin antibody is a Chicken Polyclonal antibody detecting Nestin in WB, IF and IHC (p). Suitable for Mouse and Rat.

Quick Overview for Nestin antibody (ABIN953652)

Target: Nestin (NES)

Reactivity: Mouse, Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This Nestin antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-Nestin Antibody

Specificity: Recognizes Nestin. Note that the sequences of the Mouse and Human Nestin genes are divergent, and it is unlikely that antibodies raised against the Mouse sequence would recognize Human Nestin.

Purification: Affinity Chromatography using a peptide column.

Immunogen: Three synthetic peptides KLH conjugated corresponding to different regions of the Mouse Nestin gene product (NP_057910), but are shared with the Rat (NM_012987) protein sequence. Production: After repeated injections, immune eggs were collected, and the IgY fractions were purified from the yolks. These IgY fractions were then affinity-purified using a peptide column, and the concentrations of the eluates adjusted to 0.3 mg/ml. Finally, equal volumes of each of the three affinity-purified anti-peptide antibodies were mixed, and the preparation was filter-sterilized.

Isotype: Ig Fraction

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 0.3 mg/mL

Buffer: 10 mM PBS, pH 7.2 containing 0.02 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted in the dark at 2-8 °C.

Target Details for Nestin

Target: Nestin (NES)

Alternative Name: Nestin

Background: Mouse Nestin is a 206,994 dalton protein (1864 amino acids) expressed in neural crest cells, CNS neural stems cells, as well as a few non-neural cell types in the embryo, including cells within the pancreatic islets of Langerhans and the limb bud. Nestin is an intermediate filament-associated protein, and contributes to the cytoskeleton.Synonyms: NES, Nbla00170

Gene ID: 18008

NCBI Accession: NP_057910