AHSA1 antibody

Catalog No. ABIN964557

Supplier Product No.: 200-501-974

This anti-AHSA1 antibody is a Rat Monoclonal antibody detecting AHSA1 in WB, ELISA and IHC. Suitable for Human.

Quick Overview for AHSA1 antibody (ABIN964557)

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This AHSA1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Clone: 25F2-D9

Product Details anti-AHSA1 Antibody

Supplier Product No.: 200-501-974

Supplier: Rockland

Purpose: Aha1 Antibody

Cross-Reactivity (Details): It is directed against human Aha1 protein.

Characteristics: Synonyms: rat anti-Aha1 Antibody, Aha1, Ahsa1 antibody, Activator of Hsp90 ATPase, Activator of 90 kDa heat shock protein ATPase homolog 1 antibody

Purification: This product is an IgG fraction antibody purified from tissue culture supernatant by Protein-G chromatography, followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: This Protein G purified monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse AHA1 protein followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 5-10 μg/mL

Application Note: This Protein-G purified antibody has been tested for use in ELISA, immunohistochemistry and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 38-40 kDa in size corresponding to Aha1 protein by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: None

Preservative: Without preservative

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for AHSA1

Target: AHSA1 (Activator of HSP90 ATPase Activity 1 (AHSA1))

Alternative Name: Ahsa1

Background: Background: Activator of Hsp90 ATPase (AHA1) stimulates the inherent ATPase cycle of Hsp90, which is essential for its chaperone activity in vivo.  The activation and/or stability of many of the key regulatory and signaling proteins of the eukaryotic cell depend on their interaction with the Hsp90 Molecular chaperone.  Hsp90 is assisted and regulated by co-chaperones that participate in an ordered series both to assist client-protein recruitment or release and to modulate progress through the ATPase coupled chaperone cycle.  Structural analysis and mutagenesis show that binding of the N-terminal domain of AHA1 to Hsp90 promotes a conformational switch in the middle-segment catalytic loop (aa 370-390) of Hsp90 that exposes the catalytic Arg380 and enables its interaction with ATP in the N-terminal nucleotide-binding domain of the chaperone.  Recent studies show that AHA1 modulates Hsp90-dependent stability of the folding of the cystic fibrosis transmembrane conductance regulator (CFTR) in the endoplasmic reticulum (ER).  Down-regulation of AHA1 rescues misfolding of CFTR in cystic fibrosis.

Gene ID: 217737

NCBI Accession: NP_666148

UniProt: Q8BK64