TUBA1B antibody (AA 425-451) (HRP)

Catalog No. ABIN964564

Supplier Product No.: 600-403-880

This Rabbit Polyclonal antibody specifically detects TUBA1B in WB, ELISA and IHC. It exhibits reactivity toward Human, Mouse, Rat, Chicken, Cow, Goat and Sheep.

Quick Overview for TUBA1B antibody (AA 425-451) (HRP) (ABIN964564)

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Reactivity: Human, Mouse, Rat, Chicken, Cow, Goat, Sheep

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TUBA1B antibody is conjugated to HRP

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-TUBA1B Antibody (HRP)

Binding Specificity: AA 425-451

Supplier Product No.: 600-403-880

Supplier: Rockland

Purpose: Alpha-Tubulin Antibody Peroxidase Conjugated

Cross-Reactivity (Details): Anti-Tubulin is directed against human alpha Tubulin protein.

Characteristics: Synonyms: rabbit anti-alpha-Tubulin Antibody HRP conjugation, peroxidase conjugated rabbit anti-α-tubulin antibody, peroxidase conjugated rabbit anti-alpha-Tubulin Antibody, rabbit anti-α-tubulin antibody HRP conjugation, Tubulin alpha-1B chain, Tubulin alpha-ubiquitous chain, Alpha-tubulin ubiquitous, Tubulin K-alpha-1, TUBA1B, tubulin loading control, Alpha-tubulin, Tubulin alpha-1A, TUBA1A, TUBA3, LIS3

Purification: The Tubulin Antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Optional[Immunogen]: Anti-Tubulin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the C-Terminal region near amino acids 425-451 of Human alpha Tubulin.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry Dilution: 1:500 - 1:2,500

Application Note: Anti-Tubulin Loading Control Antibody has been tested for use in ELISA and western blot.  Specific conditions for reactivity should be optimized by the end user. Expect a band at ~50 kDa in size corresponding to alpha tubulin by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:1,000 - 1:5,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Preservative: Gentamicin sulfate

Precaution of Use: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store Anti-Tubulin Loading Control Antibody at 4° C prior to restoration. For extended storage aliquot antibody and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge Loading Control if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for TUBA1B

Target: TUBA1B (Tubulin, alpha 1B (TUBA1B))

Alternative Name: TUBA1B

Background: Background: Microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Tubulin itself is a globular protein consisting of two polypeptides (alpha and beta tubulin). Alpha and beta tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter. Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase. Detyrosinated alpha tubulin is referred to as Glu-tubulin. Another post-translational modification of detyrosinated alpha tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle.  This antibody makes an excellent loading control.

UniProt: P68363

Pathways: Microtubule Dynamics, M Phase