CRASP-1 antibody
Rockland 200-401-c18
Reactivity: Borrelia burgdorferi
ELISA, WB
Host: Rabbit
Polyclonal
unconjugated
-
- Target
- CRASP-1
- Reactivity
- Borrelia burgdorferi
- Host
- Rabbit
- Clonality
- Polyclonal
- Application
- ELISA, Western Blotting (WB)
- Supplier Product No.
- 200-401-c18
- Supplier
- Rockland
- Purpose
- CRASP-1 Antibody
- Cross-Reactivity (Details)
- This antibody is specific for Borrelia burgdorferi CRASP-1 protein.
- Characteristics
- Synonyms: rabbit anti-CRASP-1 Antibody, Borrelia burgdorferi CRASP-1, CRASP1, CRASP 1, Complement regulator acquiring protein 1
- Purification
- This product was Protein-A purified and cross-adsorbed against MBP from monospecific antiserum by chromatography.
- Immunogen
-
Immunogen: MBP-fusion protein corresponding to Borrelia burgdorferi CRASP-1 protein.
Immunogen Type: Recombinant Protein
- Isotype
- IgG
-
- Application Notes
-
Application Note: This protein-A purified antibody has been tested for use in ELISA and Western blotting. Specific conditions for reactivity should be optimized by the user. Expect a band approximately 26.9 kDa in size corresponding to Borrelia burgdorferi CRASP-1 protein by Western blotting in the appropriate cell lysate or extract.
Western Blot Dilution: 1:1,000
ELISA Dilution: >1:5,000
Other: User Optimized
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
-
Reconstitution Volume: 100 μL
Reconstitution Buffer: Restore with deionized water (or equivalent)
- Concentration
- 1.0 mg/mL
- Buffer
-
Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Stabilizer: None
Preservative: 0.01 % (w/v) Sodium Azide - Preservative
- Sodium azide
- Precaution of Use
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
- Expiry Date
- 12 months
-
- Target
- CRASP-1
- Alternative Name
- CRASP1
- Background
- Background: CRASP-1, or Complement Regulator-Acquiring Surface Protein 1, is a multifunctional protein of Lyme disease-causing B. burgdorferi that binds to several human extracellular matrix proteins and plasminogen, including factor H (resulting in inhibition of complement activation in mammals) and Human Bone Morphogenic Protein 2. These interactions may contribute to adhesion, bacterial colonization, and organ tropism and may allow dissemination of B. burgdorferi in the host. B. burgdorferi spirochetes express up to 5 complement regulator-acquiring surface proteins. Multiple copies of sequences analagous to CRASP-1 genes have been detected in Borrelia plasmids. Borrelia species contain a large number of plasmids, of linear and circular, some of which appear to repeat sequences or contain fragments of other genes. These regions may serve as potentially usable information for the survival of Borrelia in its multiple environments during its life cycle. In addition, the sequence for CRASP-1 contains a repeated sequence folded into a stable stem loop structure typical of RNA genes.
- Gene ID
- 1194383
- NCBI Accession
- WP_010890397
- UniProt
- Q66ZC1
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