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Fluorescein antibody (Alkaline Phosphatase (AP))

FACS, IM Host: Goat Polyclonal Alkaline Phosphatase (AP) F(ab')2 fragment
Catalog No. ABIN964653
  • Target
    Fluorescein
    Fragment
    F(ab')2 fragment
    Host
    • 24
    • 12
    • 5
    • 4
    • 3
    • 1
    Goat
    Clonality
    • 26
    • 16
    • 7
    Polyclonal
    Conjugate
    • 26
    • 5
    • 3
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Alkaline Phosphatase (AP)
    Application
    • 22
    • 18
    • 17
    • 8
    • 8
    • 7
    • 6
    • 6
    • 6
    • 3
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Flow Cytometry (FACS), Immunomicroscopy (IM)
    Specificity
    This product was prepared from monospecific antiserum by immunoaffinity chromatography using Fluorescein IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, pepsin digestion and chromatographic separation. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase, anti-Goat Serum and Fluorescein conjugated Bovine Serum Albumin. No reaction was observed against anti-Pepsin or anti-Goat IgG F(c).
    Characteristics
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Immunogen
    Fluorescein conjugated to Goat IgG
    Immunogen Type: Other
    Isotype
    IgG
  • Application Notes
    Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1.0 mg/mL
    Buffer
    0.05 M Tris Chloride, 0.15 M Sodium Chloride, 0.005 M Magnesium Chloride, 0.0005 M Zinc Chloride, 50 % (v/v) Glycerol, pH 8.0, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. Expiration date is one (1) year from date of opening.
    Expiry Date
    12 months
  • Target
    Fluorescein
    Target Type
    Chemical
    Background
    This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
    Synonyms: FITC, ALP, Alk Phos
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