TRPC6 antibody (C-Term)

Catalog No. ABIN964697

Supplier Product No.: 200-301-b59

Product Details anti-TRPC6 Antibody

Target: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRPC6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Supplier Product No.: 200-301-b59

Supplier: Rockland

Purpose: TRPC6 Antibody

Cross-Reactivity (Details): This antibody is specific for human TRPC6 protein.

Characteristics: Synonyms: mouse anti-TRPC6 Antibody, TRPC 6, TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6

Purification: This product was purified from concentrated tissue culture supernate by Protein A chromatography.

Sterility: Sterile filtered

Immunogen:

Immunogen: This monoclonal antibody was produced by repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human TRPC6 protein.

Immunogen Type: Conjugated Peptide

Clone: 3F2-H10-F2

Isotype: IgG1 kappa

Application Details

Application Notes:

Immunohistochemistry Dilution: 2.5 μg/mL

Application Note: Anti-TRPC6 monoclonal antibody (200-301-B59) clone # 3F2.H10.F2 was developed by Rockland Immunochemicals Inc. against human TRPC6 using conventional hybridoma technology by fusing splenocytes of a host animal immunized with a synthetic peptide corresponding to the cytosolic domain of TRPC6 with myeloma cells. The screening of clones during the subcloning process was based on immunohistochemistry using human tissue microarrays. The pathologist analyzing the staining patterns of clones reported that the antibody shows strong to moderate staining consistent with the localization of human TRPC6 in adrenal cortex, neurons, Purkinje cells, colon epithelium, cardiac myocytes, renal tubules, hepatocytes, skeletal muscle, pancreatic exocrine and islet cells, germinal center lymphocytes, plasma cells, Sertoli cells of the testes as well as staining more faintly other tissues known to be positive for the target protein (e.g., respiratory epithelium). Prostate and placenta were negative for staining. The antibody produced minimal to no background staining and appeared very specific at 2.5 μg/mL. The pattern of reactivity observed for this clone was also similar to other antibodies used for benchmarking purposes. Specific conditions for reactivity should be optimized by the end user, however, we suggest the use of formalin-fixed paraffin-embedded sections for immunohistochemistry. No pre-treatment of sample is required. While immunohistochemistry was used as the primary screening and release validation immunoassay, clone #3F2.H10.F2 was also screened by western blotting against known positive and negative control lysates. A single band is detected by this antibody in TRPC6 positive cells and tissues, however, the molecular weight of the band (~30 kDa) is not consistent with full length human TRPC6 (181 kDa). The band detected by this antibody may be the cleaved cytosolic domain of TRPC6 as the immunogen used for antibody production corresponds to an amino acid sequence located within this domain. However, no additional data is available to elucidate the molecular composition of this band.

Western Blot Dilution: 1:500- 1:2,000

ELISA Dilution: 1:10,000 - 1:50,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

References for anti-TRPC6 antibody (ABIN964697)

Alonso-González, Cabo, San José, Gago, Suazo, García-Suárez, Cobo, Vega: "Human Digital Meissner Corpuscles Display Immunoreactivity for the Multifunctional Ion Channels Trpc6 and Trpv4." in: Anatomical record (Hoboken, N.J. : 2007), Vol. 300, Issue 6, pp. 1022-1031, (2018) (PubMed).

Target Details for TRPC6

Target: TRPC6 (Transient Receptor Potential Cation Channel, Subfamily C, Member 6 (TRPC6))

Alternative Name: TRPC6 (TRPC6 Products)

Background: Background: TRPC6, also known as TRP6, short transient receptor potential channel 6 and transient receptor potential cation channel subfamily C member 6, is thought to form a receptor-activated non-selective calcium permeant cation channel. TRPC6 is probably operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. It is activated by diacylglycerol (DAG) in a membrane-delimited fashion, independently of protein kinase C and may not to be activated by intracellular calcium store depletion. Defects in this gene are a cause of focal segmental glomerulosclerosis (FSGS). Expression of this protein has been reported in tissues such as placenta, lung, spleen, ovary, small intestine, and renal podocytes. Immunohistochemistry studies using polyclonal antibodies to this target have shown moderate to strong staining in cell types such as neurons, breast, respiratory, squamous and prostate epithelium, epidermis, placental trophoblasts, dendritic cells, and subsets of immune cells, and faint to moderate staining of adrenal, colon, ganglion cells, hepatocytes, heart, and testis.

Gene ID: 7225, 5730102

UniProt: Q9Y210