DYKDDDDK Tag antibody (Agarose Beads)

Catalog No. ABIN964701

Supplier Product No.: 200-350-383

This anti-DYKDDDDK Tag antibody is a Mouse Monoclonal antibody detecting DYKDDDDK Tag in WB and IP. Suitable for .

Quick Overview for DYKDDDDK Tag antibody (Agarose Beads) (ABIN964701)

Target: DYKDDDDK Tag

Reactivity: Please inquire

Host: Mouse

Clonality: Monoclonal

Conjugate: This DYKDDDDK Tag antibody is conjugated to Agarose Beads

Application: Western Blotting (WB), Immunoprecipitation (IP)

Clone: 29E4-G7

Product Details anti-DYKDDDDK Tag Antibody (Agarose Beads)

Supplier Product No.: 200-350-383

Supplier: Rockland

Purpose: DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated

Sequence: DYKDDDDK

Cross-Reactivity (Details): This product is intended for purification of proteins containing the FLAG® epitope tag sequence.

Characteristics: Synonyms: mouse anti-FLAG® tag agarose gel, mouse anti-DYKDDDDK agarose gel, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, D-Y-K-D-D-D-D-K, DDK, FLAG, FLAG antibody, anti-Flag, anti-DDK, DDK antibody

Purification: DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated is a purified mouse IgG2a monoclonal antibody coupled to activated agarose.

Immunogen:

Immunogen: DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated was produced in mice by repeated immunizations with a synthetic peptide corresponding to the FLAG® epitope tag peptide DYKDDDDK (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) conjugated to KLH.

Immunogen Type: Conjugated Peptide

Isotype: IgG2a kappa

Application Details

Application Notes:

Application Note: DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated has been tested by IP and western blot and is optimally suited for immunoprecipitation and purification of FLAG® tagged fusion proteins.DYKDDDDK Tag (Anti-FLAG®) Antibody Agarose Conjugated recognizes the FLAG® epitope tag fused to either the amino- or carboxy-terminal ends or an internal location of targeted fusion proteins. The epitope tag peptide sequence was first derived from the 11-amino-acid leader peptide of the gene-10 product from bacteriophage T7. DYKDDDDK is the most commonly used hydrophilic octapeptide tag. Use D-Y-K-D-D-D-D-K peptide (p/n 000-000-383) for competitive elution to recover fusion protein (see protocol). Anti-FLAG® is a registered trademark of Sigma-Aldrich. Refer to the protocol for complete instructions for use including preferred buffers for elution. Do not use buffers that may denature the anti-DYKDDDDK antibody.

Immunoprecipitation Dilution: 10 μL resin binds >1 μg FLAG® fusion protein

Restrictions: For Research Use only

Handling

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store vial at 4°C prior to opening.

Expiry Date: 12 months

Target Details for DYKDDDDK Tag

Target: DYKDDDDK Tag

Alternative Name: FLAG

Target Type: Tag

Background: Background: Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Anti-epitope tag antibodies serve as universal detection reagents for any tag containing protein produced by recombinant means.  Epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.