IL-2 antibody

Catalog No. ABIN964739

Supplier Product No.: 202-401-b95

Product Details anti-IL-2 Antibody

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Reactivity: Cat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IL-2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Supplier Product No.: 202-401-b95

Supplier: Rockland

Purpose: IL-2 Antibody

Cross-Reactivity (Details): This antibody is specific for cat IL-2 protein.

Characteristics: Synonyms: rabbit anti-Interleukin-2, rabbit anti-IL-2 antibody, IL2, T-cell growth factor, TCGF, Aldesleukin, Lymphokine

Purification: This product was protein A purified from monospecific antiserum by chromatography.

Immunogen:

Immunogen: This protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein raised in yeast corresponding to 134 amino acids of mature cat IL-2.

Immunogen Type: Recombinant Protein

Isotype: IgG

Application Details

Application Notes:

Application Note: This protein A purified IL-2 antibody has been tested by ELISA and Western blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 15.4 kDa in size corresponding to feline IL-2 by western blotting in the appropriate cell lysate or extract.

Western Blot Dilution: 1:500-1:2,000

ELISA Dilution: 1:20,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1.0 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for IL-2

Target: IL-2 (IL2) (Interleukin 2 (IL2))

Alternative Name: IL2 (IL2 Products)

Background: Background: IL-2 is a member of a family of cytokines, which also includes IL-4, IL-7, IL-9, IL-15 and IL-21. All members of this family signal through a receptor complex that includes the common gamma chain. IL-2 signals through a receptor complex consisting of IL-2 specific IL-2 receptor alpha (CD25), IL-2 receptor beta (CD122) and the common gamma chain. The main function of IL-2 is to promote the development of T regulatory cells. IL-2 is produced by several cell types including, B cells, gamma-delta T cells, CD4+ T cells, CD8+ T cells, and eosinophils. It has been found to be similar to IL-15 in terms of function. Both IL-2 and IL-15 are able to facilitate production of immunoglobulins made by B cells and induce the differentiation and proliferation of natural killer cells. The primary differences between IL-2 and IL-15 are found in adaptive immune responses: IL-2 participates in maintenance of T-Regs and reduces self-reactive T cells while IL-15 is necessary for maintaining highly specific T cell responses by supporting survival of CD8 memory T cells.

Gene ID: 751114

NCBI Accession: NP_001036802

UniProt: Q07885

Pathways: JAK-STAT Signaling, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Activated T Cell Proliferation