Interleukin 17a antibody

Catalog No. ABIN964796

Supplier Product No.: 210-501-b32s

The Rat Monoclonal anti-Interleukin 17a antibody has been validated for ELISA, WB, FACS and MA. It is suitable to detect Interleukin 17a in samples from Mouse.

Quick Overview for Interleukin 17a antibody (ABIN964796)

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Interleukin 17a antibody is un-conjugated

Application: ELISA, Western Blotting (WB), Flow Cytometry (FACS), Multiplex Assay (MA)

Clone: 20B4-G10-F5

Product Details anti-Interleukin 17a Antibody

Supplier Product No.: 210-501-b32s

Supplier: Rockland

Purpose: Mouse IL-17A Antibody

Cross-Reactivity (Details): This antibody is specific for mouse IL-17A protein.

Characteristics: Synonyms: rat anti-IL-17A antibody, rat anti-interleukin17A antibody, Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8

Purification: This monoclonal antibody is purified by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen:

Immunogen: This Mouse IL-17A monoclonal antibody was produced in rats by repeated immunizations with full length recombinant mouse IL-17A protein (produced in E.coli) followed by hybridoma development.

Immunogen Type: Recombinant Protein

Isotype: IgG2a kappa

Application Details

Application Notes:

Flow Cytometry Dilution: 1:500

Application Note: IL-17 A antibody has been tested for use in western blotting, Flow Cytometry and ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 23 kDa in size corresponding to the mature mouse IL-17A protein, a non-glycosylated polypeptide chain consisting of 207 amino acids, by western blotting in appropriate cell lysate or extract.

Western Blot Dilution: 1:1000

ELISA Dilution: 1:10,000

Other: User Optimized

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 2.94 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store IL-17A antibody at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Interleukin 17a

Target: Interleukin 17a (IL17A) (Interleukin 17A (IL17A))

Alternative Name: IL17a

Background: Background: Mouse Interleukin-17A (IL-17A), also known as CTLA-8, is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Mouse IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58 % identity between the amino acid sequence of human and mouse IL-17A. Anti-IL-17A antibody is ideal for investigators involved in Immunology research.

Gene ID: 16171

NCBI Accession: NP_034682

UniProt: Q62386