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beta Actin antibody (N-Term)

Name: beta Actin antibody (N-Term)
Brand: antibodies-online
SKU: ABIN965658
Price: 495 USD
Availability: InStock

ACTB Reactivity: Human WB, IHC (p) Host: Mouse Monoclonal ac-15 unconjugated

Lane 1: Rat Ovary Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: Rat Cardiac Muscle Tissue LysateLane 4: Rat Brain Tissue LysateLane 5: A453 Cell LysateLane 6: HELA Cell LysateLane 7: SMMC Cell LysateLane 8: HT1080 Cell LysateLane 9: SW620 Cell Lysate (beta Actin antibody (N-Term))

Lane 1: Rat Liver Tissue LysateLane 2: Rat Spleen Tissue LysateLane 3: Rat Brain Tissue LysateLane 4: Rat Kidney Tissue LysateLane 5: HELA Cell LysateLane 6: SMMC Cell LysateLane 7: SGC Cell Lysate (beta Actin antibody (N-Term))

2 images

(2 references)

Catalog No. ABIN965658

Datasheet as PDF

Target See all beta Actin (ACTB) Antibodies

beta Actin (ACTB) (Actin, beta (ACTB))
Binding Specificity
41

16

15

6

6

4

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1
N-Term
Reactivity
178

131

111

55

42

42

37

27

25

23

18

17

12

10

8

7

7

6

6

6

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1
Human
Host
113

81

5

1

1
Mouse
Compatible Secondaries
Clonality
112

87
Monoclonal
Conjugate
127

12

12

10

3

3

3

3

3

3

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This beta Actin antibody is un-conjugated
Application
168

74

52

46

33

31

26

21

13

13

8

6

6

6

5

2

1

1

1
Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Cross-Reactivity

Mouse (Murine), Rat (Rattus)

Cross-Reactivity (Details)

No cross reactivity with other proteins.

Immunogen

slightly modified beta-cytoplasmic actin N-terminal peptide, Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys, conjugated to KLH.

Clone

Ac-15

Isotype

IgG1

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ACTB Reactivity: Mouse, Rat, Chicken, Zebrafish (Danio rerio) WB, IHC, ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Rat WB, IF, IHC (p), FACS, DNA Mic Host: Mouse Monoclonal 137CT26-1-1 unconjugated

anti-Actin, beta (ACTB) (AA 350-375) antibody
ACTB Reactivity: Human, Mouse, Leopard Frog WB, ELISA, IF, FM Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) (AA 1-25) antibody
ACTB Reactivity: Human, Mouse, Rat, Pig, Cow WB, IHC (p) Host: Mouse Monoclonal 1A2 unconjugated

anti-Actin, beta (ACTB) (AA 1-375) antibody
ACTB Reactivity: Human WB, ELISA, IHC (p) Host: Mouse Monoclonal 3G4-F9 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
KO Validated ACTB Reactivity: Human WB, IF, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Actin, beta (ACTB) antibody
ACTB Reactivity: Human, Mouse, Chicken, Rabbit, Pig, Cow, Hamster, Goat, Fish WB, ELISA Host: Mouse Monoclonal 2D1D10 unconjugated

anti-Actin, beta (ACTB) (AA 1-50) antibody
ACTB Reactivity: Human WB, ELISA, IHC, IF, IP, FACS Host: Mouse Monoclonal 1D12E12B5 unconjugated

anti-Actin, beta (ACTB) (N-Term) antibody
ACTB Reactivity: Human, Mouse, Rat, Chicken, Rabbit, Dog, Monkey, Pig, Cow, Sheep, Hamster, Horse, Guinea Pig, Goat, Cat, Avian, Donkey WB, IF Host: Goat Polyclonal unconjugated

Restrictions

For Research Use only
Format

Lyophilized

Reconstitution

2.2 % sodium acetate or neutral PBS. If 1 mL of PBS is used, the antibody concentration will be 100 µg/mL.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice

Avoid repeated freezing and thawing.

Storage

-20 °C

Storage Comment

At -20 °C for one year. After reconstitution, at 4 °C for one month. It can also be aliquotted and stored frozen at -20 °C for a longer time.
Ng, Gunning, Eddy, Ponte, Leavitt, Shows, Kedes: "Evolution of the functional human beta-actin gene and its multi-pseudogene family: conservation of noncoding regions and chromosomal dispersion of pseudogenes." in: Molecular and cellular biology, Vol. 5, Issue 10, pp. 2720-32, (1986) (PubMed).

Tardieu, Virot, Vandroux, Vergnoux, Doumeix, Delhoume, Chabanier, Blanc, Richard, Bensaid: "[A case of electrically and mechanically stunned myocardium]." in: Archives des maladies du coeur et des vaisseaux, Vol. 78, Issue 6, pp. 947-50, (1985) (PubMed).
Target

beta Actin (ACTB) (Actin, beta (ACTB))

Alternative Name

beta-Actin (ACTB Products)

Background

The primary site of action of cytochalasin B on cell motility processes is beta-actin. Habets et al. (1992) generated hybrids that harbor only specific regions of human chromosome 7 and assigned the ACTB locus to 7p15-p12. ACTB and the other assigned beta-actin-related sequences are dispersed over at least four different chromosomes including one locus assigned to the X chromosome. A mutation of beta-actin that alters depolymerization dynamics is associated with autosomal dominant developmental malformations, deafness, and dystonia.

Gene ID

60, 11461, 81822

UniProt

P60710, P60711, P60709

Pathways

Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Maintenance of Protein Location, Phototransduction