ABO (Blood Group Antigen B) antibody

Catalog No. ABIN965678

There are 3+ publications for this product available. The Mouse Monoclonal anti-ABO (Blood Group Antigen B) antibody has been validated for IHC (fp), IHC (p) and IHC (fro). It is suitable to detect ABO (Blood Group Antigen B) in samples from Human.

Quick Overview for ABO (Blood Group Antigen B) antibody (ABIN965678)

Target: ABO (Blood Group Antigen B)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B460

Product Details

Specificity: This antibody stains erythrocytes and vascular epthelium of blood group B controls.

Purification: Concentrated.

Immunogen: Blood Group B erythrocytes.

Isotype: IgM

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
Staining Procedure: This antibody can be used on frozen and formalin-fixed paraffin-embedded tissue sections. When using on formalin-fixed paraffin embedded tissues a antigen demasking procedure is recommended.The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Human Tissue (Blood Group B or AB)

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Ricard, Boboyono, Postel, Collet, Canarelli: "[Gastric rupture in children. Apropos of a case]." in: Annales de pédiatrie, Vol. 36, Issue 7, pp. 479-81, (1989) (PubMed).

Chapman, Allhoff, Proppe, Prout: "Use of monoclonal antibodies for the localization of tissue isoantigens A and B in transitional cell carcinoma of the upper urinary tract." in: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, Vol. 31, Issue 4, pp. 557-61, (1983) (PubMed).

Bundle, Gidney, Kassam, Rahman: "Hybridomas specific for carbohydrates; synthetic human blood group antigens for the production, selection, and characterization of monoclonal typing reagents." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 129, Issue 2, pp. 678-2, (1982) (PubMed).

Target Details

Target: ABO (Blood Group Antigen B)

Alternative Name: Blood Group Antigen B

Gene ID: 4059