CAPN1 antibody (AA 312-326)

Catalog No. ABIN965738

The Polyclonal anti-CAPN1 antibody has been validated for WB and IHC (p). It is suitable to detect CAPN1 in samples from Human, Mouse and Rat. There is 1 publication available.

Quick Overview for CAPN1 antibody (AA 312-326) (ABIN965738)

Target: CAPN1 (Calpain 1 (CAPN1))

Reactivity: Human, Mouse, Rat

Host: Please inquire

Clonality: Polyclonal

Conjugate: This CAPN1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CAPN1 Antibody

Binding Specificity: AA 312-326

Immunogen: A synthetic peptide corresponding to a sequence at the middle region of human Calpain-1 (312-326 aa), different from the mouse sequence by two amino acids.

Application Details

Restrictions: For Research Use only

Handling

Reconstitution: 0.2ml of distilled water will yield a concentration of 500µg/ml.

Buffer: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4

Preservative: Thimerosal (Merthiolate), Sodium azide

Storage: -20 °C

References for anti-CAPN1 antibody (ABIN965738)

Imajoh, Aoki, Ohno, Emori, Kawasaki, Sugihara, Suzuki: "Molecular cloning of the cDNA for the large subunit of the high-Ca2+-requiring form of human Ca2+-activated neutral protease." in: Biochemistry, Vol. 27, Issue 21, pp. 8122-8, (1989) (PubMed).

Target Details for CAPN1

Target: CAPN1 (Calpain 1 (CAPN1))

Alternative Name: Calpain 1

Background: Calpain-1 catalytic subunit is a protein that in humans is encoded by the CAPN1 gene. Calpain is an intracellular protease that requires calcium for its catalytic activity. Two isozymes, calpain I (mu-calpain) and calpain II (m-calpain), with different calcium requirements, have been identified. Both are heterodimers composed of L (large, catalytic, 80 kD) and S (small, regulatory, 30 kD) subunits. The isozymes share an identical S subunit, with the differences arising from the L subunits, L1 (CAPN1) and L2. By quantitative RT-PCR, Ueyama et al. (1998) found that expression of calpain-1 and calpain-2 mRNA was significantly increased in muscle biopsy samples derived from 5 men with progressive muscular dystrophy (e.g., DMD, 310200) and 2 men and 3 women with amyotrophic lateral sclerosis (ALS, 105400) compared with controls. Using cDNA clones as probes, Ohno et al. (1989, 1990) assign CANPL1 to chromosome 11.