CD13 Myeloid Cell antibody

Catalog No. ABIN965764

This Mouse Monoclonal antibody specifically detects in FACS, IHC (af), IHC (fro) and IF. It exhibits reactivity toward Human and has been mentioned in 3+ publications.

Quick Overview for CD13 Myeloid Cell antibody (ABIN965764)

Target: CD13 Myeloid Cell

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Acetone-fixed) (IHC (af)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF)

Clone: B347 (B-F10)

Product Details

Specificity: This antibody reacts with CD13, a 150 kD cell surface glycoprotein. The CD13 antigen is identical to aminopeptidase N. The specificity of these antibodies are similar to those of WM-15 and My7.

Purification: Purified.

Immunogen: Human KG-1 (myeloid) cultured cells.

Isotype: IgG1 kappa

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL.
Flow cytometry: 1-5 ug/10^6 cells.
Immunohistochemistry: 5-20 ug/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on acetone fixed frozen cryostat sections.

Comment:

Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Look, Ashmun, Shapiro, Peiper: "Human myeloid plasma membrane glycoprotein CD13 (gp150) is identical to aminopeptidase N." in: The Journal of clinical investigation, Vol. 83, Issue 4, pp. 1299-307, (1989) (PubMed).

Sakai, Hattori, Sagawa, Yokoyama, Takatsuki: "Biochemical and functional characterization of MCS-2 antigen (CD13) on myeloid leukemic cells and polymorphonuclear leukocytes." in: Cancer research, Vol. 47, Issue 21, pp. 5572-6, (1987) (PubMed).

Look, Peiper, Rebentisch, Ashmun, Roussel, Rettenmier, Sherr: "Transfer and expression of the gene encoding a human myeloid membrane antigen (gp150)." in: The Journal of clinical investigation, Vol. 75, Issue 2, pp. 569-79, (1985) (PubMed).

Target Details

Target: CD13 Myeloid Cell

Alternative Name: CD13,Myeloid Cell

Gene ID: 290