CD20 (B Cells) (Extracellular Domain) antibody

Catalog No. ABIN965777

The Mouse Monoclonal anti- antibody has been validated for FACS, IF and IHC (fro). It is suitable to detect in samples from Human. There are 2+ publications available.

Quick Overview for CD20 (B Cells) (Extracellular Domain) antibody (ABIN965777)

Target: CD20 (B Cells)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B342 (B9E9)

Product Details

Binding Specificity: Extracellular Domain

Specificity: This antibody reacts with an antigen present on most of the B-cells. They react with a 33 kD polypeptide and a minor component of a 30 kD polypeptide present in B-cells.

Purification: Purified.

Immunogen: Lymphoblastoid cell line DAUDI.

Isotype: IgG2a

Application Details

Application Notes: Immunofluorescence: 5-20 ug/mL
Flow cytometry: 1-5 ug/10^6 cells
Western blotting: 1-5 ug/mL.
Immunohistochemistry: 5-20 mg/mL on frozen sections using avidin-biotin system. This antibody was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen cryostat sections.

Comment:

Cellular Localization: Cell membrane.
Recommended Positive Control: Tonsil and Lymph Node

Restrictions: For Research Use only

Handling

Concentration: 0.2 mg/ml.

Buffer: 50 mM Sodium phosphate, 150 mM Sodium Chloride, BSA 0.2%, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Kanzaki, Shibata, Mogami, Kojima: "Expression of calcium-permeable cation channel CD20 accelerates progression through the G1 phase in Balb/c 3T3 cells." in: The Journal of biological chemistry, Vol. 270, Issue 22, pp. 13099-104, (1995) (PubMed).

Bubien, Zhou, Bell, Frizzell, Tedder: "Transfection of the CD20 cell surface molecule into ectopic cell types generates a Ca2+ conductance found constitutively in B lymphocytes." in: The Journal of cell biology, Vol. 121, Issue 5, pp. 1121-32, (1993) (PubMed).

Target Details

Target: CD20 (B Cells)

Molecular Weight: 33-37 kDa

Gene ID: 931