CD3 antibody

Catalog No. ABIN965782

This Mouse Monoclonal antibody specifically detects CD3 in FACS, IHC (fro), IF and WB. It exhibits reactivity toward Human.

Quick Overview for CD3 antibody (ABIN965782)

Target: CD3

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Clone: B477 (33-2A3)

Product Details anti-CD3 Antibody

Specificity: This antibody reacts with the CD3 antigen of the TCR/CD3 complex.

Purification: Purified.

Immunogen: Human leucocytes

Isotype: IgG2a

Application Details

Application Notes: Immunofluorescence: 5-20ug/ml.
Immunohistochemistry: 5-20gu/ml on frozen and formalin-fixed paraffinembedded tissue sections using avidin-biotin system.
Flow cytometry: 1-5ug/10^6 cells.
Western blotting: 15ug/ml. An antigen demasking procedure is recommended when using formalinfixed paraffin-embedded tissue sections. This MAb is clustered in the 6th International Leucocytes Workshop on human leucocyte differentiation antigens in Kobe, Japan.
Staining Procedure: This antibody can be used on frozen cryostat sections.

Comment:

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Target Details for CD3

Target: CD3

Alternative Name: CD3

Pathways: TCR Signaling, Ubiquitin Proteasome Pathway