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CD3 antibody

CD3 Reactivity: Human FACS, IHC (fro), IF, WB Host: Mouse Monoclonal B477 (33-2A3) unconjugated

Catalog No. ABIN965782

Datasheet as PDF

Target See all CD3 Antibodies

CD3
Reactivity
182

104

26

20

17

10

8

8

7

7

5

3

2

1
Human
Host
217

53

20

13

9

2
Mouse
Compatible Secondaries
Clonality
302

6

1
Monoclonal
Conjugate
95

39

32

23

23

11

9

8

6

5

5

5

4

4

3

3

3

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
This CD3 antibody is un-conjugated
Application
287

82

57

52

46

36

32

23

9

8

7

6

4

3

2

2

2

2

2

2

2

2

1

1

1
Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunofluorescence (IF), Western Blotting (WB)

Specificity

This antibody reacts with the CD3 antigen of the TCR/CD3 complex.

Purification

Purified.

Immunogen

Human leucocytes

Clone

B477 (33-2A3)

Isotype

IgG2a

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CD3 Reactivity: Human FACS, IP Host: Mouse Monoclonal MEM-57 Biotin

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CD3 Reactivity: Human FACS Host: Mouse Monoclonal MEM-57 APC

Application Notes

Immunofluorescence: 5-20ug/ml.
Immunohistochemistry: 5-20gu/ml on frozen and formalin-fixed paraffinembedded tissue sections using avidin-biotin system.
Flow cytometry: 1-5ug/10^6 cells.
Western blotting: 15ug/ml. An antigen demasking procedure is recommended when using formalinfixed paraffin-embedded tissue sections. This MAb is clustered in the 6th International Leucocytes Workshop on human leucocyte differentiation antigens in Kobe, Japan.
Staining Procedure: This antibody can be used on frozen cryostat sections.

Comment

Recommended Positive Control: Tonsil, Thymus, Spleen

Restrictions

For Research Use only
Format

Liquid

Concentration

0.4 mg/ml.

Buffer

50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C
Target

CD3

Alternative Name

CD3 (CD3 Products)

Pathways

TCR Signaling, Ubiquitin Proteasome Pathway