CD69 antibody

Catalog No. ABIN965813

The Mouse Monoclonal anti-CD69 antibody has been validated for FACS, WB, IHC and ELISA. It is suitable to detect CD69 in samples from Human. There are 4+ publications available.

Quick Overview for CD69 antibody (ABIN965813)

Target: CD69

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD69 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunohistochemistry (IHC), ELISA

Clone: 8B6

Product Details anti-CD69 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CD69 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: Western Bloting: 1/500 - 1/2000.
Immunohistochemistry: 1/200 - 1/1000.
Flow cytometry: 1/200 - 1/400.
ELISA: Propose dilution 1/10000.
Not yet tested in other applications.
Determining optimal working dilutions by titration test.

Restrictions: For Research Use only

Handling

Format: Liquid

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD69 antibody (ABIN965813)

Vance, Harley, Backlund, Ward, Phelps, Gress: "Human CD69 associates with an N-terminal fragment of calreticulin at the cell surface." in: Archives of biochemistry and biophysics, Vol. 438, Issue 1, pp. 11-20, (2005) (PubMed).

Moore: "Seeking alternatives to euthanasia." in: Journal of the American Veterinary Medical Association, Vol. 220, Issue 1, pp. 20; author reply 20, (2003) (PubMed).

Atzeni, Schena, Ongari, Carrabba, Bonara, Minonzio, Capsoni: "Induction of CD69 activation molecule on human neutrophils by GM-CSF, IFN-gamma, and IFN-alpha." in: Cellular immunology, Vol. 220, Issue 1, pp. 20-9, (2003) (PubMed).

Iafrate, Bronson, Skowronski: "Separable functions of Nef disrupt two aspects of T cell receptor machinery: CD4 expression and CD3 signaling." in: The EMBO journal, Vol. 16, Issue 4, pp. 673-84, (1997) (PubMed).

Target Details for CD69

Target: CD69

Alternative Name: CD69

Background: Involved in lymphocyte proliferation and functions as a signal transmitting receptor in lymphocytes, natural killer (NK) cells, andplatelets Subcellular location: Membrane, Single-pass type II membrane protein Tissue specificity: Expressed on the surface of activated T-cells, B-cells, natural killer cells, neutrophils, eosinophils, epidermal Langerhanscells and platelets
Sequence similarities: Contains 1 C-type lectin domain.
Synonyms: CLEC2C, CD69

Molecular Weight: 22.5kDa

Gene ID: 969