CD8 alpha antibody

Catalog No. ABIN965822

The Mouse Monoclonal anti-CD8 alpha antibody has been validated for FACS, IHC (fro) and IHC (af). It is suitable to detect CD8 alpha in samples from Human. There are 2+ publications available.

Quick Overview for CD8 alpha antibody (ABIN965822)

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD8 alpha antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro)), Immunohistochemistry (Acetone-fixed) (IHC (af))

Clone: B441 (143-44 )

Product Details anti-CD8 alpha Antibody

Specificity: This antibody consists of a disufide-linked / hetrodimer with a mW of 30 kD or an / homodimer with a MW of 32 kD. The CD molecule binds to HLA class I molecule during interaction of CD 8+ T cells with antigenpresenting cells or with target cells. CD 8+T cells include most of the cytotoxic T cells.

Characteristics: This antibody is directed against alpha chain of CD8 molecule.

Purification: Purified.

Isotype: IgG kappa

Application Details

Application Notes: Flow: not tested in our lab.
IHC (acetone-fixed frozen).
Staining Procedure: Acetone-fixed tissue sections at 1:50-1:100 Antibody

Comment:

Cellular Localization: Cell membrane.
Recommended Positive Control: Human peripheral lymphocytes, Human tonsil.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.2 mg/0.5 ml.

Buffer: Protein A/G purified, 0.2 mg in 50 mM Borate, 150mM NaCl, 20 % Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD8 alpha antibody (ABIN965822)

Akimoto, Kaneko, Sekigawa, Hashimoto, Kaneko, Yamamoto: "Binding of HIV-2 envelope glycoprotein to CD8 molecules and related chemokine production." in: Immunology, Vol. 95, Issue 2, pp. 214-8, (1999) (PubMed).

Washizuka, Aizawa, Chinushi, Naitoh, Miyajima, Kusano, Kitazawa, Uchiyama, Takahashi, Shibata: "Alternation of QRS morphology and effect of radiofrequency ablation in idiopathic ventricular tachycardia." in: Pacing and clinical electrophysiology : PACE, Vol. 18, Issue 1 Pt 1, pp. 18-27, (1995) (PubMed).

Target Details for CD8 alpha

Target: CD8 alpha (CD8A) (CD8a Molecule (CD8A))

Alternative Name: CD8a

Molecular Weight: 32 kDa

Gene ID: 925

Pathways: TCR Signaling