GNRHR antibody (Extracellular Domain)

Catalog No. ABIN966217

This Mouse Monoclonal antibody specifically detects GNRHR in WB, ELISA, FACS and IHC (fro). It exhibits reactivity toward Human and has been mentioned in 4+ publications.

Quick Overview for GNRHR antibody (Extracellular Domain) (ABIN966217)

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GNRHR antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B470

Product Details anti-GNRHR Antibody

Binding Specificity: AA 1-29, Extracellular Domain

Specificity: This antibody reacts with GnrH receptors found in the anterior pituitary. GnrH functions as a stimulator of gonadotrophs to release luteinizing hormone (LH) and follicle stimulating hormone (FSH).

Purification: Concentrated.

Immunogen: BSA-conjugated peptide (amino acids1-29) of human GnrH receptor extracellular domain.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100.
ELISA: 1:1000-1:3000.
Flow cytometry: 1:150-1:300.
Western Blot 1:200-1:500.
Staining Procedure: This antibody can be used on frozen tissue sections. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Pituitary Tissue.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/ml.

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-GNRHR antibody (ABIN966217)

Grosse, Schöneberg, Schultz, Gudermann: "Inhibition of gonadotropin-releasing hormone receptor signaling by expression of a splice variant of the human receptor." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 9, pp. 1305-18, (1997) (PubMed).

Jennes, Eyigor, Janovick, Conn: "Brain gonadotropin releasing hormone receptors: localization and regulation." in: Recent progress in hormone research, Vol. 52, pp. 475-90; discussion 490-1, (1997) (PubMed).

Fraser, Sellar, Illingworth, Eidne: "GnRH receptor mRNA expression by in-situ hybridization in the primate pituitary and ovary." in: Molecular human reproduction, Vol. 2, Issue 2, pp. 117-21, (1997) (PubMed).

Arora, Cheng, Catt: "Mutations of the conserved DRS motif in the second intracellular loop of the gonadotropin-releasing hormone receptor affect expression, activation, and internalization." in: Molecular endocrinology (Baltimore, Md.), Vol. 11, Issue 9, pp. 1203-12, (1997) (PubMed).

Target Details for GNRHR

Target: GNRHR (Gonadotropin-Releasing Hormone Receptor (GNRHR))

Alternative Name: GnRH Receptor

Gene ID: 2798