CD81 antibody

Catalog No. ABIN966327

Product Details anti-CD81 Antibody

Target: CD81

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD81 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF), Immunohistochemistry (Frozen Sections) (IHC (fro))

Specificity: This antibody reacts with a 24 kD protein, a member of the tetraspan family, with both termini in the cytoplasm and two loops of extracellular protein. This protein lacks external N-glycosylation sites.

Purification: Purified.

Immunogen: Burkitt's lymphoma cell line.

Clone: B399 (1-3-3-22)

Isotype: IgG1

Application Details

Application Notes: Immunofluorescence: 10-20 ug/mL
Flow cytometry: 1ug / 10^6 cells
Immunohistochemistry: 10-20 ug/mL on acetone-fixed frozen sections.
This product was not quality controlled in flow cytometry.
Staining Procedure: This antibody can be used on frozen tissue sections only, in IHC. On formalin-fixed, paraffin embedded tissues, some positive staining is observed when treated with a high temperature antigenic unmasking techniques Used for identification of eosinophils from a granulocyte subpopulation. The optimum dilution factors should be determined by the individual laboratory.

Comment:

Recommended Positive Control: Tonsil for immunohistochemistry

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.4 mg/ml.

Buffer: 50 mM Sodium Borate, 150 mM Sodium Chloride, 20% Glycerol, pH 8.0

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-CD81 antibody (ABIN966327)

Schick, Levy: "The TAPA-1 molecule is associated on the surface of B cells with HLA-DR molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 151, Issue 8, pp. 4090-7, (1993) (PubMed).

Bradbury, Kansas, Levy, Evans, Tedder: "The CD19/CD21 signal transducing complex of human B lymphocytes includes the target of antiproliferative antibody-1 and Leu-13 molecules." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 149, Issue 9, pp. 2841-50, (1992) (PubMed).

Levy, Nguyen, Andria, Takahashi: "Structure and membrane topology of TAPA-1." in: The Journal of biological chemistry, Vol. 266, Issue 22, pp. 14597-602, (1991) (PubMed).

Oren, Takahashi, Doss, Levy, Levy: "TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins." in: Molecular and cellular biology, Vol. 10, Issue 8, pp. 4007-15, (1990) (PubMed).

Target Details for CD81

Target: CD81

Alternative Name: CD81 (CD81 Products)

Gene ID: 975

Pathways: Inositol Metabolic Process, Hepatitis C