MUC5AC antibody

Catalog No. ABIN966608

The Mouse Monoclonal anti-MUC5AC antibody has been validated for ELISA, IHC (p) and IHC (fro). It is suitable to detect MUC5AC in samples from Human, Mouse, Rat, Monkey, Rabbit, Chicken, Pig and Hedgehog. There are 3+ publications available.

Quick Overview for MUC5AC antibody (ABIN966608)

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Reactivity: Human, Mouse, Rat, Monkey, Rabbit, Chicken, Pig, Hedgehog

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC5AC antibody is un-conjugated

Application: ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: B466(45M1)

Product Details anti-MUC5AC Antibody

Specificity: This antibody reacts with the peptide core of gastric mucin (MUC5AC).

Purification: Concentrated.

Immunogen: Ovarian cyst mucin.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry: 1:50-1:100
ELISA 1:500-1:2000 (not purified can not be used to coat ELISA plates).
Staining Procedure: This antibody can be used on frozen and formalin-fixed, paraffin-embedded tissue sections. For consistant and better results, use protease treatment with formalin fixed tissues. Prolonged-fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50-1:100 in IHC. The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: cytoplasmic and cell surface.
Recommended Positive Control: Colon or Stomach Tissue

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.3 mg/mL

Buffer: 20 mM tris-borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing fetal bovine serum, BMC-6 carrier polysaccharides, carrier protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References for anti-MUC5AC antibody (ABIN966608)

Rickert, Piller, Henkel, Fromme, Lieleg: "Multifunctional glycoprotein coatings improve the surface properties of highly oxygen permeable contact lenses." in: Biomaterials advances, Vol. 145, pp. 213233, (2022) (PubMed).

Rickert, Lutz, Marczynski, Lieleg: "Several Sterilization Strategies Maintain the Functionality of Mucin Glycoproteins." in: Macromolecular bioscience, pp. e2000090, (2020) (PubMed).

Lutz, Marczynski, Grill, Wall, Lieleg: "Repulsive Backbone-Backbone Interactions Modulate Access to Specific and Unspecific Binding Sites on Surface-Bound Mucins." in: Langmuir : the ACS journal of surfaces and colloids, (2020) (PubMed).

Target Details for MUC5AC

Target: MUC5AC (Mucin 5AC, Oligomeric Mucus/gel-Forming (MUC5AC))

Alternative Name: MUC5AC

Background: Peptide core of gastric mucin M1, Epitope destroyed by beta-mercaptoethanol and proteases but not by periodate (3).

Molecular Weight: >1000kDa

Gene ID: 4586