NEUROFILAMENTS antibody

Catalog No. ABIN966644

Product Details

Target: NEUROFILAMENTS

Reactivity: Human, Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Application: Immunohistochemistry (Formalin-fixed Paraffin-embedded Sections) (IHC (fp)), Immunoprecipitation (IP), Western Blotting (WB)

Purification: Concentrated.

Immunogen: Neurofilament from Human Brain

Clone: 2F11

Isotype: IgG1 kappa

Application Details

Application Notes: Immunohistochemistry: (Paraffin/Frozen): 1:50-1:100
WB: 1:100-1:200.
Staining Procedure: Prolonged fixation in buffered formalin can destroy the epitope. The antibody may be used at a dilution of 1:50 to 1:100 in IHC . The optimal conditions should be determined by the individual laboratory.

Comment:

Cellular Localization: Cytoplasmic.
Recommended Positive Control: Human brain, IMR-5 cells

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/ml.

Buffer: 20 mM Tris-Borate, 150 mM Sodium Chloride, dialyzed media RPMI 1640/D-MEM containing Fetal Bovine Serum, BMC-6 Carrier Polysaccharides, Carrier Protein, pH 7.5

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

References

Baldinger: "Sodium hyaluronate used to correct lens nucleus tumble." in: Ophthalmic surgery, Vol. 16, Issue 9, pp. 590, (1985) (PubMed).

Target Details

Target: NEUROFILAMENTS

Background: This antibody reacts with the 70 and 200 kD polypeptides of neurofilament. This antibody recognizes neurons, neuronal processes, and peripheral nerves, as well as, sympathetic ganglion cells and adrenal medulla. Cell body of neurons containing the non-phosphorylated neurofilamentis weakly stained.

Molecular Weight: 68 kDa, 200 kDa(phosphorylated form)