HSP70 antibody

Catalog No. ABIN967447

Product Details anti-HSP70 Antibody

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP)

Brand: BD Pharmingen™

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Purified Hsp70

Clone: 5G10

Isotype: IgG2b

Application Details

Application Notes: HeLa cervical carcinoma cells (ATCC CCL-2) are suggested as a positive control.

Comment:

Related Products: ABIN968535, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-HSP70 antibody (ABIN967447)

Cyr, Langer, Douglas: "DnaJ-like proteins: molecular chaperones and specific regulators of Hsp70." in: Trends in biochemical sciences, Vol. 19, Issue 4, pp. 176-81, (1994) (PubMed).

Craig, Gross: "Is hsp70 the cellular thermometer?" in: Trends in biochemical sciences, Vol. 16, Issue 4, pp. 135-40, (1991) (PubMed).

Singh, Yu: "Accumulation of a heat shock-like protein during differentiation of human erythroid cell line K562." in: Nature, Vol. 309, Issue 5969, pp. 631-3, (1984) (PubMed).

Target Details for HSP70

Target: HSP70 (Heat Shock Protein 70 (HSP70))

Alternative Name: Hsp70 (HSP70 Products)

Background: Cells from many organisms increase the expression of heat shock or stress proteins in response to adverse changes. The heat shock protein (HSP) 70 family consists of multiple members, localized within different intracellular compartments. HSP 70 is constitutively expressed in many human cells with increased expression observed following stress. Members of the HSP 70 family bind ATP, and are thought to function as molecular chaperones by assisting in the folding of other proteins in various intracellular compartments. HSP 70 migrates at a molecular weight of 70 kDa in SDS-PAGE. The 5G10 antibody recognizes human HSP 70. Purified HSP 70 was isolated from K562, a human erythroid cell line, and used as immunogen.

Molecular Weight: 70 kDa