GFAP antibody

Catalog No. ABIN967499

The Mouse Monoclonal anti-GFAP antibody has been validated for WB, IF and IHC (f). It is suitable to detect GFAP in samples from Human, Rat, Mouse, Pig, Cow, Chicken, Rabbit, Guinea Pig, Sheep and Dog. There are 5+ publications available.

Quick Overview for GFAP antibody (ABIN967499)

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Reactivity: Human, Rat, Mouse, Pig, Cow, Chicken, Rabbit, Guinea Pig, Sheep, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This GFAP antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Formalin-fixed Sections) (IHC (f))

Clone: 4A11

Product Details anti-GFAP Antibody

Brand: BD Pharmingen™

Cross-Reactivity: Human, Mouse (Murine), Rat (Rattus), Sheep (Ovine), Dog (Canine), Pig (Porcine), Rabbit, Guinea Pig, Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Cow spinal cord homogenate

Isotype: IgG2b

Application Details

Application Notes: Applications include immunohistochemical staining of formalin-fixed paraffin-embedded brain tissue sections (25 µg/ml). Other applications not routinely tested include indirect immunofluorescence of tissue-cultured cells and western blot analysis (1-2 µg/ml). Rat brain is suggested as a positive control.

Comment:

Related Products: ABIN967501, ABIN967500, ABIN967502

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.5 mg/mL

Buffer: Aqueous buffered solution containing ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store undiluted at 4°C.

References for anti-GFAP antibody (ABIN967499)

Schwartz, Cheng, Mughal, Mattson, Yao: "Clathrin assembly proteins AP180 and CALM in the embryonic rat brain." in: The Journal of comparative neurology, Vol. 518, Issue 18, pp. 3803-18, (2010) (PubMed).

Graziano, Liu, Murray, Jones: "Vesicular glutamate transporters define two sets of glutamatergic afferents to the somatosensory thalamus and two thalamocortical projections in the mouse." in: The Journal of comparative neurology, Vol. 507, Issue 2, pp. 1258-76, (2008) (PubMed).

McLendon, Bigner: "Immunohistochemistry of the glial fibrillary acidic protein: basic and applied considerations." in: Brain pathology (Zurich, Switzerland), Vol. 4, Issue 3, pp. 221-8, (1994) (PubMed).

McLendon, Burger, Pegram, Eng, Bigner: "The immunohistochemical application of three anti-GFAP monoclonal antibodies to formalin-fixed, paraffin-embedded, normal and neoplastic brain tissues." in: Journal of neuropathology and experimental neurology, Vol. 45, Issue 6, pp. 692-703, (1986) (PubMed).

Pegram, Eng, Wikstrand, McComb, Lee, Bigner: "Monoclonal antibodies reactive with epitopes restricted to glial fibrillary acidic proteins of several species." in: Neurochemical pathology, Vol. 3, Issue 2, pp. 119-38, (1985) (PubMed).

Target Details for GFAP

Target: GFAP (Glial Fibrillary Acidic Protein (GFAP))

Alternative Name: GFAP

Background: GFAP (Glial Fibrillary Acid Protein) is the major protein of glial filaments in differentiated astrocytes. We offer a panel of monoclonal antibodies (4A11, 1B4, 2E1) that specifically recognize GFAP. They do not cross-react with other intermediate filaments such as vimentin, neurofilament proteins, desmin, keratin, neurotubules or microfilaments. Clone 4A11 is specific for GFAP. Bovine spinal cord homogenate was used as immunogen. This antibody has broad species reactivity, recognizing GFAP in brain homogenates from human, mouse, rat, bovine, ovine, canine, porcine, rabbit, guinea pig and chicken. 4A11 is particularly useful for identifying GFAP in immunohistochemistry of frozen and formalin-fixed, paraffin-embedded brain tissue sections. Additional applications include western blot analysis and indirect immunofluorescence of tissue-cultured cells. We offer additional GFAP specific antibodies: clone 2E1 (ABIN967501), 1B4 (ABIN967500), and clones 1B4, 4A11, 2E1 combined and available as a cocktail” (ABIN967502).

Molecular Weight: 50 kDa