PKC iota antibody (AA 397-558)

Catalog No. ABIN967826

The Mouse Monoclonal anti-PKC iota antibody has been validated for WB, IHC, IP and IF. It is suitable to detect PKC iota in samples from Human, Mouse, Rat, Dog and Chicken. There are 5+ publications available.

Quick Overview for PKC iota antibody (AA 397-558) (ABIN967826)

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC iota antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 41-PKClambda

Product Details anti-PKC iota Antibody

Binding Specificity: AA 397-558

Cross-Reactivity: Rat (Rattus), Dog (Canine), Mouse (Murine), Chicken

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human PKClambda aa. 397-558

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968545

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-PKC iota antibody (ABIN967826)

Trauzold, Wermann, Arlt, Schütze, Schäfer, Oestern, Röder, Ungefroren, Lampe, Heinrich, Walczak, Kalthoff: "CD95 and TRAIL receptor-mediated activation of protein kinase C and NF-kappaB contributes to apoptosis resistance in ductal pancreatic adenocarcinoma cells." in: Oncogene, Vol. 20, Issue 31, pp. 4258-69, (2001) (PubMed).

Pauken, Capco: "The expression and stage-specific localization of protein kinase C isotypes during mouse preimplantation development." in: Developmental biology, Vol. 223, Issue 2, pp. 411-21, (2000) (PubMed).

Jain, Zhang, Kee, Li, Cao: "Protein kinase C delta associates with and phosphorylates Stat3 in an interleukin-6-dependent manner." in: The Journal of biological chemistry, Vol. 274, Issue 34, pp. 24392-400, (1999) (PubMed).

Uberall, Giselbrecht, Hellbert, Fresser, Bauer, Gschwendt, Grunicke, Baier: "Conventional PKC-alpha, novel PKC-epsilon and PKC-theta, but not atypical PKC-lambda are MARCKS kinases in intact NIH 3T3 fibroblasts." in: The Journal of biological chemistry, Vol. 272, Issue 7, pp. 4072-8, (1997) (PubMed).

Akimoto, Mizuno, Osada, Hirai, Tanuma, Suzuki, Ohno: "A new member of the third class in the protein kinase C family, PKC lambda, expressed dominantly in an undifferentiated mouse embryonal carcinoma cell line and also in many tissues and cells." in: The Journal of biological chemistry, Vol. 269, Issue 17, pp. 12677-83, (1994) (PubMed).

Target Details for PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: Protein Kinase C lambda

Background: The Protein Kinase C (PKC) family of homologous serine/threonine protein kinases is involved in a number of processes such as growth, differentiation, and cytokine secretion. At least eleven isozymes have been described. These proteins are products of multiple genes and alternative splicing. PKC consists of a single polypeptide chain containing four conserved regions (C) and five variable regions (V). The N-terminal half containing C1, C2, V1, and V2 constitutes the regulatory domain and interacts with the PKC activators Ca2+, phospholipid, diacylglycerol, or phorbol ester. However, the novel PKC (nPKC) subfamily members ( delta, epsilon, eta, and theta isoforms) and the atypical PKC (aPKC) subfamily members (zeta, ί, and lambda isoforms) are Ca2+ independent and lack the C2 domain. The aPKC members are unique in that their activity is independent of diacylglycerols and phorbol esters. They also lack one repeat of the cysteine-rich sequences that are conserved in cPKC and nPKC. The C-terminal region of PKC contains the catalytic domain. The PKC pathway represents a major signal transduction system that is activated following ligand-stimulation of transmembrane receptors by hormones, neurotransmitters, and growth factors. PKClambda shows the highest degree of amino acid homology with PKCzeta (72%) and PKClambda mRNA is expressed in a variety of cells and tissues. The PKClambda protein kinase is capable of autophosphorylation and can be activated by phosphatidylserine, but not by other PKC activators such as diacylglycerols, Ca2+, or phorbol esters.

Molecular Weight: 74 kDa

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation