Dynamin 1 antibody (AA 698-851)

Catalog No. ABIN967852

This Mouse Monoclonal antibody specifically detects Dynamin 1 in WB, IHC, IF and IP. It exhibits reactivity toward Human, Mouse, Rat, Dog and Chicken and has been mentioned in 5+ publications.

Quick Overview for Dynamin 1 antibody (AA 698-851) (ABIN967852)

Target: Dynamin 1 (DNM1)

Reactivity: Human, Mouse, Rat, Dog, Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This Dynamin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP)

Clone: 41-Dynamin

Product Details anti-Dynamin 1 Antibody

Binding Specificity: AA 698-851

Cross-Reactivity: Human, Dog (Canine), Chicken, Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Rat Dynamin I aa. 698-851

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN968539, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Dynamin 1 antibody (ABIN967852)

Hayes, Chawla, Corvera: "TGF beta receptor internalization into EEA1-enriched early endosomes: role in signaling to Smad2." in: The Journal of cell biology, Vol. 158, Issue 7, pp. 1239-49, (2002) (PubMed).

Kalthoff, Groos, Kohl, Mahrhold, Ungewickell: "Clint: a novel clathrin-binding ENTH-domain protein at the Golgi." in: Molecular biology of the cell, Vol. 13, Issue 11, pp. 4060-73, (2002) (PubMed).

Schmidlin, Dery, DeFea, Slice, Patierno, Sternini, Grady, Bunnett: "Dynamin and Rab5a-dependent trafficking and signaling of the neurokinin 1 receptor." in: The Journal of biological chemistry, Vol. 276, Issue 27, pp. 25427-37, (2001) (PubMed).

Ochoa, Slepnev, Neff, Ringstad, Takei, Daniell, Kim, Cao, McNiven, Baron, De Camilli: "A functional link between dynamin and the actin cytoskeleton at podosomes." in: The Journal of cell biology, Vol. 150, Issue 2, pp. 377-89, (2000) (PubMed).

Scaife, Gout, Waterfield, Margolis: "Growth factor-induced binding of dynamin to signal transduction proteins involves sorting to distinct and separate proline-rich dynamin sequences." in: The EMBO journal, Vol. 13, Issue 11, pp. 2574-82, (1994) (PubMed).

Target Details for Dynamin 1

Target: Dynamin 1 (DNM1)

Alternative Name: Dynamin

Background: Dynamin is a nerve terminal phosphoprotein with intrinsic GTPase activity which plays an important role in endocytosis. This GTPase activity is stimulated in vitro by microtubules, SH3 domain-containing proteins, phospholipids, and Protein Kinase C (PKC)-mediated phosphorylation. There are at least two distinct dynamin genes in mammals, which show 79% identity, encoding proteins Dynamin I and Dynamin II. Dynamin I is expressed almost exclusively in the central nervous system while Dynamin II expression is ubiquitous. Depolarization stimulates synaptic vesicle recycling and Dynamin I is subsequently dephosphorylated. This rapid dephosphorylation is mediated by calcineurin which acts as a switch for depolarization-initiated synaptic vesicle endocytosis. The C-terminus of dynamin contains two clusters of proline-rich SH3 domain binding proline motifs which interact with known SH3 domain proteins during tyrosine kinase receptor activation. It is reported that clone 41 detects both dynamin I and dynamin II.

Molecular Weight: 100 kDa

Pathways: Toll-Like Receptors Cascades, CXCR4-mediated Signaling Events, Thromboxane A2 Receptor Signaling