Gelsolin antibody (AA 592-768)

Catalog No. ABIN967953

This anti-Gelsolin antibody is a Mouse Monoclonal antibody detecting Gelsolin in WB, IHC, IP and IF. Suitable for Human, Rat, Mouse, Rabbit and Dog. This Primary Antibody has been cited in 5+ publications.

Quick Overview for Gelsolin antibody (AA 592-768) (ABIN967953)

Target: Gelsolin (GSN)

Reactivity: Human, Rat, Mouse, Rabbit, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This Gelsolin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: 2-Gelsolin

Product Details anti-Gelsolin Antibody

Binding Specificity: AA 592-768

Cross-Reactivity: Dog (Canine), Mouse (Murine), Rabbit, Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human Gelsolin aa. 592-768

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN968536, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-Gelsolin antibody (ABIN967953)

Wang, Xie, Du, Wu, Feng, Mei, McDonald, Xiong: "Regulation of the formation of osteoclastic actin rings by proline-rich tyrosine kinase 2 interacting with gelsolin." in: The Journal of cell biology, Vol. 160, Issue 4, pp. 565-75, (2003) (PubMed).

Arnt, Chiorean, Heldebrant, Gores, Kaufmann: "Synthetic Smac/DIABLO peptides enhance the effects of chemotherapeutic agents by binding XIAP and cIAP1 in situ." in: The Journal of biological chemistry, Vol. 277, Issue 46, pp. 44236-43, (2002) (PubMed).

De Botton, Sabri, Daugas, Zermati, Guidotti, Hermine, Kroemer, Vainchenker, Debili: "Platelet formation is the consequence of caspase activation within megakaryocytes." in: Blood, Vol. 100, Issue 4, pp. 1310-7, (2002) (PubMed).

Slee, Adrain, Martin: "Executioner caspase-3, -6, and -7 perform distinct, non-redundant roles during the demolition phase of apoptosis." in: The Journal of biological chemistry, Vol. 276, Issue 10, pp. 7320-6, (2001) (PubMed).

Kwiatkowski, Stossel, Orkin, Mole, Colten, Yin: "Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain." in: Nature, Vol. 323, Issue 6087, pp. 455-8, (1986) (PubMed).

Target Details for Gelsolin

Target: Gelsolin (GSN)

Alternative Name: Gelsolin

Background: Gelsolin was identified as a result of its ability to sever actin filaments in a Ca2+-dependent manner. The gene for gelsolin encodes an 83 kDa protein that migrates as a 93 kDa polypeptide in SDS-gels. The N-terminal domain contains the calcium-independent actin-severin site, whereas the calcium-dependent site is located in the C-terminal portion of the protein. It exhibits significant homology with villin, another calcium-regulated actin filament severing protein. Gelsolin can be found intracellularly, as well as in a secreted form. However, both forms are encoded by the same gene. This antibody is routinely tested by western blot analysis.

Molecular Weight: 93 kDa

Pathways: Caspase Cascade in Apoptosis, Regulation of Actin Filament Polymerization, Autophagy