Cadherin 4 antibody (AA 22-201)

Catalog No. ABIN967954

This Mouse Monoclonal antibody specifically detects Cadherin 4 in WB, IHC and IP. It exhibits reactivity toward Mouse and Rat and has been mentioned in 3+ publications.

Quick Overview for Cadherin 4 antibody (AA 22-201) (ABIN967954)

Target: Cadherin 4 (CDH4)

Reactivity: Mouse, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cadherin 4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Clone: 48-R

Product Details anti-Cadherin 4 Antibody

Binding Specificity: AA 22-201

Cross-Reactivity: Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse R-Cadherin aa. 22-201

Isotype: IgG2a kappa

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Cadherin 4 antibody (ABIN967954)

Tanihara, Sano, Heimark, St John, Suzuki: "Cloning of five human cadherins clarifies characteristic features of cadherin extracellular domain and provides further evidence for two structurally different types of cadherin." in: Cell adhesion and communication, Vol. 2, Issue 1, pp. 15-26, (1995) (PubMed).

Hutton, Christofori, Chi, Edman, Guest, Hanahan, Kelly: "Molecular cloning of mouse pancreatic islet R-cadherin: differential expression in endocrine and exocrine tissue." in: Molecular endocrinology (Baltimore, Md.), Vol. 7, Issue 9, pp. 1151-60, (1994) (PubMed).

Matsunami, Miyatani, Inoue, Copeland, Gilbert, Jenkins, Takeichi: "Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene." in: Journal of cell science, Vol. 106 ( Pt 1), Issue 6, pp. 401-9, (1994) (PubMed).

Target Details for Cadherin 4

Target: Cadherin 4 (CDH4)

Alternative Name: R-Cadherin

Background: R-Cadherin was first isolated from chicken retina, but was subsequently shown to be present and highly conserved in a variety of tissues from several different species. A 913 amino acid polypeptide, R-Cadherin is highly homologous to Cadherin-4, but is most closely related to another family member, N-Cadherin. In experiments using chimeric L cells expressing chicken or mouse Cadherins, those cells expressing mouse or chicken R-Cadherin were able to aggregate with each other as well as with cells expressing N-Cadherin. However, there was no aggregation of R-Cadherin expressing cells, with P- or E-Cadherin expressing cells. This suggests that the roles of specific cadherins are conserved among types in the determination of heterogenous cell segregation and distribution during tissue morphogenesis and maintenance.

Molecular Weight: 100 kDa

Pathways: Cell-Cell Junction Organization, Regulation of Cell Size