TEAD1 antibody (AA 86-199)

Catalog No. ABIN968252

The Mouse Monoclonal anti-TEAD1 antibody has been validated for WB and IF. It is suitable to detect TEAD1 in samples from Human and Mouse. There are 5+ publications available.

Quick Overview for TEAD1 antibody (AA 86-199) (ABIN968252)

Target: TEAD1 (TEA Domain Family Member 1 (SV40 Transcriptional Enhancer Factor) (TEAD1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This TEAD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 31-TEF

Product Details anti-TEAD1 Antibody

Binding Specificity: AA 86-199

Cross-Reactivity: Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human TEF-1 aa. 86-199

Isotype: IgG1

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-TEAD1 antibody (ABIN968252)

Ueyama, Zhu, Valenzuela, Suzow, Stewart: "Identification of the functional domain in the transcription factor RTEF-1 that mediates alpha 1-adrenergic signaling in hypertrophied cardiac myocytes." in: The Journal of biological chemistry, Vol. 275, Issue 23, pp. 17476-80, (2000) (PubMed).

Deshpande, Chopra, Rangarajan, Shashidhara, Rodrigues, Krishna: "The human transcription enhancer factor-1, TEF-1, can substitute for Drosophila scalloped during wingblade development." in: The Journal of biological chemistry, Vol. 272, Issue 16, pp. 10664-8, (1997) (PubMed).

Gupta, Amin, Gupta, Hay, Zak: "Transcription enhancer factor 1 interacts with a basic helix-loop-helix zipper protein, Max, for positive regulation of cardiac alpha-myosin heavy-chain gene expression." in: Molecular and cellular biology, Vol. 17, Issue 7, pp. 3924-36, (1997) (PubMed).

Jacquemin, Hwang, Martial, Dollé, Davidson: "A novel family of developmentally regulated mammalian transcription factors containing the TEA/ATTS DNA binding domain." in: The Journal of biological chemistry, Vol. 271, Issue 36, pp. 21775-85, (1996) (PubMed).

Xiao, Davidson, Matthes, Garnier, Chambon: "Cloning, expression, and transcriptional properties of the human enhancer factor TEF-1." in: Cell, Vol. 65, Issue 4, pp. 551-68, (1991) (PubMed).

Target Details for TEAD1

Target: TEAD1 (TEA Domain Family Member 1 (SV40 Transcriptional Enhancer Factor) (TEAD1))

Alternative Name: TEF-1

Background: TEF-1 (transcription enhancer factor-1) is a member of a family of transcription factors that contain an evolutionarily conserved DNA binding domain (DBD), TEA/ATTS. TEF-1 was identified as a HeLa cell transcriptional activator that bound, via its TEA domain, to the GT-IIC and Sph enhancer elements. Comparison of various enhancers indicates that TEF-1 binds to highly degenerate DNA sequences and cooperatively interacts with tandem repeats of its binding sites, which increases its enhancer activity. However, the DNA binding activities of TEF-1 are modulated by sequences outside the DBD. The N-terminal basic portion of TEF-1 contains the DBD. The C-terminal portion contains the transactivation domain and a zinc finger-like motif that is thought to be involved in protein dimerization or interaction with additional transcription factors. TEF-1 induced transcription requires a limiting transcriptional intermediary factor (TIF) and a TATA-binding protein associated factor (hTAFIIs). Although TEF-1 is expressed ubiquitously during embryogenesis, its expression is limited to adult kidney, heart, brain, skeletal muscle, and lungs. Disruption of murine TEF-1 gene results in fetal death.

Molecular Weight: 53 kDa

Pathways: Regulation of Lipid Metabolism by PPARalpha