CD51 antibody (AA 609-722)

Catalog No. ABIN968314

This Mouse Monoclonal antibody specifically detects CD51 in WB and IF. It exhibits reactivity toward Human, Mouse, Rat and Dog and has been mentioned in 3+ publications.

Quick Overview for CD51 antibody (AA 609-722) (ABIN968314)

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD51 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 21-CD51

Product Details anti-CD51 Antibody

Binding Specificity: AA 609-722

Cross-Reactivity: Rat (Rattus), Dog (Canine), Mouse (Murine)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human CD51/VNRalpha aa. 609-722

Isotype: IgG1 kappa

Application Details

Comment:

Related Products: ABIN968545, ABIN967389

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20° C.

References for anti-CD51 antibody (ABIN968314)

Bodary, McLean: "The integrin beta 1 subunit associates with the vitronectin receptor alpha v subunit to form a novel vitronectin receptor in a human embryonic kidney cell line." in: The Journal of biological chemistry, Vol. 265, Issue 11, pp. 5938-41, (1990) (PubMed).

Suzuki, Argraves, Arai, Languino, Pierschbacher, Ruoslahti: "Amino acid sequence of the vitronectin receptor alpha subunit and comparative expression of adhesion receptor mRNAs." in: The Journal of biological chemistry, Vol. 262, Issue 29, pp. 14080-5, (1987) (PubMed).

Suzuki, Argraves, Pytela, Arai, Krusius, Pierschbacher, Ruoslahti: "cDNA and amino acid sequences of the cell adhesion protein receptor recognizing vitronectin reveal a transmembrane domain and homologies with other adhesion protein receptors." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 83, Issue 22, pp. 8614-8, (1986) (PubMed).

Target Details for CD51

Target: CD51 (ITGAV) (Integrin alpha V (ITGAV))

Alternative Name: CD51

Background: Integrins are a family of proteins that mediate intercellular adhesion or adherence to extracellular matrix proteins. Their roles are essential for embryonic development, tumor metastasis, organ function, and proper immune cell function. Family members are heterodimers that contain a larger alpha subunit that is unique to each individual receptor and a smaller beta subunit that can be shared by several receptors. Based on beta subunit content, integrins are divided into the subfamilies beta1, beta2, and beta3. The beta3 subfamily contains the vitronectin receptor (alphaVbeta3) and the platelet protein gpIIb/IIIa. The alpha chain of the vitronectin receptor (VNRalpha, CD51, integrin alphaV) has been reported to consist of a disulfide-linked large extracellular (125 kDa) subunit and a smaller (25 kDa) membrane-anchored subunit. The large subunit contains multiple sequences with homology to calcium-binding sites in other proteins and an RGD-dependent ligand-binding site, while the small subunit contains a transmembrane domain and a short cytoplasmic domain. VNRalpha has been reported to be noncovalently associated with the VNR beta3-chain (CD61, gpIIIa). The VNR mediates cell adhesion to RGD-containing ligands such as vitronectin, von Willebrand factor, fibrinogen, and thrombospondin. This antibody is routinely tested by western blot analysis.
Synonyms: Integrin alphaV, Vitronectin Receptor alpha, VNRalpha

Molecular Weight: 125 kDa

Pathways: Cell-Cell Junction Organization, Signaling Events mediated by VEGFR1 and VEGFR2, Growth Factor Binding, Integrin Complex