Aldehyde Dehydrogenase antibody (AA 7-128)

Catalog No. ABIN968411

The Mouse Monoclonal anti-Aldehyde Dehydrogenase antibody has been validated for WB, IF and IHC. It is suitable to detect Aldehyde Dehydrogenase in samples from Human. There are 4+ publications available.

Quick Overview for Aldehyde Dehydrogenase antibody (AA 7-128) (ABIN968411)

Target: Aldehyde Dehydrogenase (ALDH)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Aldehyde Dehydrogenase antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC)

Clone: 44-ALDH

Product Details anti-Aldehyde Dehydrogenase Antibody

Binding Specificity: AA 7-128

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
3. Source of all serum proteins is from USDA inspected abattoirs located in the United States.
4. Please refer to us for technical protocols.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Human ALDH1 aa. 7-128

Isotype: IgG1

Application Details

Comment:

Related Products: ABIN967389, ABIN968533

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-Aldehyde Dehydrogenase antibody (ABIN968411)

Zhou, Hanna, Roberts, Weber, Bell: "ALDH1 immunohistochemical expression and its significance in salivary adenoid cystic carcinoma." in: Head & neck, Vol. 35, Issue 4, pp. 575-8, (2013) (PubMed).

Greene, Bahn, Masson, Rabbitts: "The T-cell oncogenic protein HOX11 activates Aldh1 expression in NIH 3T3 cells but represses its expression in mouse spleen development." in: Molecular and cellular biology, Vol. 18, Issue 12, pp. 7030-7, (1998) (PubMed).

Yoshida, Rzhetsky, Hsu, Chang: "Human aldehyde dehydrogenase gene family." in: European journal of biochemistry / FEBS, Vol. 251, Issue 3, pp. 549-57, (1998) (PubMed).

Kathmann, Lipsky: "Cloning of a cDNA encoding a constitutively expressed rat liver cytosolic aldehyde dehydrogenase." in: Biochemical and biophysical research communications, Vol. 236, Issue 2, pp. 527-31, (1997) (PubMed).

Target Details for Aldehyde Dehydrogenase

Target: Aldehyde Dehydrogenase (ALDH)

Alternative Name: ALDH

Background: Aldehyde dehydrogenase (ALDH) is a ubiquitous enzyme located in nearly all mammalian tissues. It catalyzes the irreversible oxidation of a range of aliphatic and aromatic aldehydes to their corresponding carboxylic acids. There are multiple isoforms of ALDH which are subdivided into three classes. Class I includes the cytosolic isoforms. Class II includes the mitochondrial isoforms. Class III includes the microsomal, cytosolic tumor specific, and cytosolic dioxin-inducible forms. At least twelve human ALDH isoforms have been identified. Mutations of many of these proteins such as ALDH1, ALDH2, ALDH4, and ALDH10 have been implicated in multiple human metabolic disorders and clinical abnormalities. At the amino acid level, human ALDH isoforms exhibit a wide range of diversity (15% to about 80%). However, multiple protein regions have been highly conserved and are important for functional activities. A well-characterized member of the human ALDH family is ALDH1. It plays a major role in the biosynthesis of retinoic acid from retinol (vitamin A). Retinoic acid, the biologically active form of retinol, is a regulator of cellular proliferation, differentiation, and survival.

Molecular Weight: 55 kDa