STX8 antibody (AA 57-164)

Catalog No. ABIN968489

The Mouse Monoclonal anti-STX8 antibody has been validated for WB and IF. It is suitable to detect STX8 in samples from Human, Mouse, Rat and Dog. There are 2+ publications available.

Quick Overview for STX8 antibody (AA 57-164) (ABIN968489)

Target: STX8 (Syntaxin 8 (STX8))

Reactivity: Human, Mouse, Rat, Dog

Host: Mouse

Clonality: Monoclonal

Conjugate: This STX8 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 48-Syntaxin 8

Product Details anti-STX8 Antibody

Binding Specificity: AA 57-164

Cross-Reactivity: Human, Dog (Canine), Rat (Rattus)

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Immunogen: Mouse Syntaxin 8 aa. 57-164

Isotype: IgG2a

Application Details

Comment:

Related Products: ABIN967389, ABIN968536

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References for anti-STX8 antibody (ABIN968489)

Steegmaier, Yang, Yoo, Huang, Shen, Yu, Luo, Scheller: "Three novel proteins of the syntaxin/SNAP-25 family." in: The Journal of biological chemistry, Vol. 273, Issue 51, pp. 34171-9, (1999) (PubMed).

Thoreau, Bergès, Callebaut, Guillier-Gencik, Gressin, Bernheim, Karst, Mornon, Kitzis, Chomel: "Molecular cloning, expression analysis, and chromosomal localization of human syntaxin 8 (STX8)." in: Biochemical and biophysical research communications, Vol. 257, Issue 2, pp. 577-83, (1999) (PubMed).

Target Details for STX8

Target: STX8 (Syntaxin 8 (STX8))

Alternative Name: Syntaxin 8

Background: Eukaryotic protein trafficking involves the packaging of molecules into membranous vesicles that bud from a donor compartment, travel to a specific destination, fuse, and release their components into an acceptor compartment. Recognition between vesicle and acceptor membrane is mediated by the pairing of the integral membrane SNARE proteins. The stable interaction between vesicle proteins (v-SNAREs) and target proteins (t-SNAREs) juxtaposes the membranes and results in an activated docking state and/or membrane fusion. The syntaxin protein family contains a number of members that serve as functional t-SNAREs. One member of this family, syntaxin 8, has two coiled-coil domains, one in the N-terminus and one toward the C-terminus. The C-terminal domain is homologous to a similar domain in syntaxin 6 and is predicted to interact in the formation of SNARE complexes, while a hydrophobic domain in the C-terminus may be involved in membrane anchoring. Syntaxin-8 is ubiquitously expressed, but is present at high levels in the heart and localizes specifically to the ER. Thus, syntaxin-8 is a t-SNARE that is thought to be involved in the early secretory pathway of many different cell types.

Molecular Weight: 27 kDa

Pathways: Synaptic Vesicle Exocytosis