Phosphoserine/Threonine antibody

Catalog No. ABIN968874

This anti- antibody is a Mouse Monoclonal antibody detecting in WB and IF. Suitable for Human and Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for Phosphoserine/Threonine antibody (ABIN968874)

Target: Phosphoserine/Threonine

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Clone: 22A-pSer-Thr

Product Details

Characteristics: 1. Since applications vary, each investigator should titrate the reagent to obtain optimal results.
2. Please refer to us for technical protocols.
3. Caution: Sodium azide yields highly toxic hydrazoic acid under acidic conditions. Dilute azide compounds in running water before discarding to avoid accumulation of potentially explosive deposits in plumbing.
4. Source of all serum proteins is from USDA inspected abattoirs located in the United States.

Purification: The monoclonal antibody was purified from tissue culture supernatant or ascites by affinity chromatography.

Isotype: IgG1

Application Details

Application Notes: For western blotting, a dilution of 1:2500 may be a useful starting concentration for titrations.

Comment:

Related Products: ABIN968533, ABIN968887, ABIN967389, ABIN968871

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 250 μg/mL

Buffer: Aqueous buffered solution containing BSA, glycerol, and ≤0.09 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store undiluted at -20°C.

References

Brivanlou, Darnell: "Signal transduction and the control of gene expression." in: Science (New York, N.Y.), Vol. 295, Issue 5556, pp. 813-8, (2002) (PubMed).

Pawson, Scott: "Signaling through scaffold, anchoring, and adaptor proteins." in: Science (New York, N.Y.), Vol. 278, Issue 5346, pp. 2075-80, (1998) (PubMed).

Yan, Packer, Gooley, Williams: "Protein phosphorylation: technologies for the identification of phosphoamino acids." in: Journal of chromatography. A, Vol. 808, Issue 1-2, pp. 23-41, (1998) (PubMed).

Target Details

Target: Phosphoserine/Threonine

Background: Protein phosphorylation of serine and threonine residues is critical for the control of protein activity involved in various cellular events. An assortment of Ser/Thr kinases and phosphatases regulate serine and threonine phosphorylation in cell signaling pathways, such as growth factor, cytokine, chemokine, and stress response. Detection of serine and threonine phosphorylation can generally be monitored by antibodies that detect phosphoserine and phosphothreonine.