LIN28A antibody (AA 93-209)

Catalog No. ABIN968936

The Mouse Monoclonal anti-LIN28A antibody has been validated for ELISA and ICC. It is suitable to detect LIN28A in samples from Human. There are 2+ publications available.

Quick Overview for LIN28A antibody (AA 93-209) (ABIN968936)

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LIN28A antibody is un-conjugated

Application: ELISA, Immunocytochemistry (ICC)

Clone: 6D1F9

Product Details anti-LIN28A Antibody

Binding Specificity: AA 93-209

Purpose: LIN28 Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of LIN28 (aa93-209) expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-LIN28A antibody (ABIN968936)

Gerecht-Nir, Dazard, Golan-Mashiach, Osenberg, Botvinnik, Amariglio, Domany, Rechavi, Givol, Itskovitz-Eldor: "Vascular gene expression and phenotypic correlation during differentiation of human embryonic stem cells." in: Developmental dynamics : an official publication of the American Association of Anatomists, Vol. 232, Issue 2, pp. 487-97, (2005) (PubMed).

Wu, Belasco: "Micro-RNA regulation of the mammalian lin-28 gene during neuronal differentiation of embryonal carcinoma cells." in: Molecular and cellular biology, Vol. 25, Issue 21, pp. 9198-208, (2005) (PubMed).

Target Details for LIN28A

Target: LIN28A (Lin-28 Homolog A (C. Elegans) (LIN28A))

Alternative Name: LIN28

Background: LIN28: lin-28 homolog (C. elegans), also known as CSDD1, ZCCHC1. Entrez Protein NP_078950. LIN28 was first discovered in the nematode C. elegans. It is a heterochronic protein in C. elegans involved in the timing of developmental events and choice of stage specific cell fates. LIN28 expression has been found to be regulated post-transcriptionally by miRNAs in both nematodes and mammals. In humans it is expressed in embryonic stem cells and its expression decreases during differentiation. It is negatively regulated by retinoic acid in neuronal differentiation.

Molecular Weight: 23 kDa

Gene ID: 79727

UniProt: Q9H9Z2

Pathways: Stem Cell Maintenance