B-Cell Linker antibody

Catalog No. ABIN968984

The Mouse Monoclonal anti-B-Cell Linker antibody has been validated for WB, ELISA, IHC, FACS and ICC. It is suitable to detect B-Cell Linker in samples from Human and Mouse. There are 2+ publications available.

Quick Overview for B-Cell Linker antibody (ABIN968984)

Target: B-Cell Linker (BLNK)

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This B-Cell Linker antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 5G9

Product Details anti-B-Cell Linker Antibody

Purpose: BLNK Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human BLNK expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

ELISA: 1/10000

FCM: 1/200 - 1/400

ICC: 1/200 - 1/1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-B-Cell Linker antibody (ABIN968984)

Imamura, Oda, Katahira, Bundo, Pike, Ratcliffe, Kitamura: "BLNK binds active H-Ras to promote B cell receptor-mediated capping and ERK activation." in: The Journal of biological chemistry, Vol. 284, Issue 15, pp. 9804-13, (2009) (PubMed).

Kamino, Futamura, Nakamura, Kitamura, Kabu, Arakawa: "B-cell linker protein prevents aneuploidy by inhibiting cytokinesis." in: Cancer science, Vol. 99, Issue 12, pp. 2444-54, (2008) (PubMed).

Target Details for B-Cell Linker

Target: B-Cell Linker (BLNK)

Alternative Name: BLNK

Background: This gene encodes a cytoplasmic linker or adaptor protein that plays a critical role in B cell development. This protein bridges B cell receptor-associated kinase activation with downstream signaling pathways, thereby affecting various biological functions. The phosphorylation of five tyrosine residues is necessary for this protein to nucleate distinct signaling effectors following B cell receptor activation. Mutations in this gene cause hypoglobulinemia and absent B cells, a disease in which the pro- to pre-B-cell transition is developmentally blocked. Deficiency in this protein has also been shown in some cases of pre-B acute lymphoblastic leukemia. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 68 kDa

UniProt: Q8WV28

Pathways: BCR Signaling