CTCF antibody

Catalog No. ABIN969071

Product Details anti-CTCF Antibody

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Reactivity: Human, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This CTCF antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Purpose: CTCF Antibody

Purification: Ascitic fluid

Immunogen: Purified recombinant fragment of human CTCF expressed in E. Coli.

Clone: 1D11

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/10000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Ascitic fluid containing 0.03 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

References for anti-CTCF antibody (ABIN969071)

Grbesa, Marinkovic, Ivkic, Kruslin, Novak-Kujundzic, Pegan, Bogdanovic, Bedekovic, Gall-Troselj: "Loss of imprinting of IGF2 and H19, loss of heterozygosity of IGF2R and CTCF, and Helicobacter pylori infection in laryngeal squamous cell carcinoma." in: Journal of molecular medicine (Berlin, Germany), Vol. 86, Issue 9, pp. 1057-66, (2008) (PubMed).

Majumder, Gomez, Chadwick, Boss: "The insulator factor CTCF controls MHC class II gene expression and is required for the formation of long-distance chromatin interactions." in: The Journal of experimental medicine, Vol. 205, Issue 4, pp. 785-98, (2008) (PubMed).

Stedman, Kang, Lin, Kissil, Bartolomei, Lieberman: "Cohesins localize with CTCF at the KSHV latency control region and at cellular c-myc and H19/Igf2 insulators." in: The EMBO journal, Vol. 27, Issue 4, pp. 654-66, (2008) (PubMed).

Target Details for CTCF

Target: CTCF (CCCTC-Binding Factor (Zinc Finger Protein) (CTCF))

Alternative Name: CTCF (CTCF Products)

Background: This gene is a member of the BORIS + CTCF gene family and encodes a transcriptional regulator protein with 11 highly conserved zinc finger (ZF) domains. This nuclear protein is able to use different combinations of the ZF domains to bind different DNA target sequences and proteins. Depending upon the context of the site, the protein can bind a histone acetyltransferase (HAT)-containing complex and function as a transcriptional activator or bind a histone deacetylase (HDAC)-containing complex and function as a transcriptional repressor. If the protein is bound to a transcriptional insulator element, it can block communication between enhancers and upstream promoters, thereby regulating imprinted expression. Mutations in this gene have been associated with invasive breast cancers, prostate cancers, and Wilms' tumors.Tissue specificity: Ubiquitous. Absent in primary spermatocytes.

Molecular Weight: 83 kDa

UniProt: P49711